Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC

被引：179

作者：

Harrison, Ewan M. ^{[1
]}

Paterson, Gavin K. ^{[1
]}

Holden, Matthew T. G. ^{[2
]}

Larsen, Jesper ^{[3
]}

Stegger, Marc ^{[3
]}

Larsen, Anders Rhod ^{[3
]}

Petersen, Andreas ^{[3
]}

Skov, Robert L. ^{[3
]}

Christensen, Judit Marta ^{[4
]}

Zeuthen, Anne Bak ^{[4
]}

Heltberg, Ole ^{[4
]}

Harris, Simon R. ^{[2
]}

Zadoks, Ruth N. ^{[5
]}

Parkhill, Julian ^{[2
]}

Peacock, Sharon J. ^{[2
,6
]}

Holmes, Mark A. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Vet Med, Cambridge, England

[2] Wellcome Trust Sanger Inst, Hinxton, England

[3] Statens Serum Inst, DK-2300 Copenhagen, Denmark

[4] Dept Clin Microbiol, Slagelse Sygehus, Denmark

[5] Moredun Res Inst, Penicuik, Midlothian, Scotland

[6] Univ Cambridge, Dept Clin Med, Cambridge, England

来源：

EMBO MOLECULAR MEDICINE | 2013年 / 5卷 / 04期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

cattle; mecC; MRSA; sheep; zoonosis; RESISTANT STAPHYLOCOCCUS-AUREUS; EVOLUTION; CONTRIBUTES; VIRULENCE; CARRIAGE; ANIMALS; ADHESIN; PROTEIN; ORIGIN;

D O I：

10.1002/emmm.201202413

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Several methicillin-resistant Staphylococcus aureus (MRSA) lineages that carry a novel mecA homologue (mecC) have recently been described in livestock and humans. In Denmark, two independent human cases of mecC-MRSA infection have been linked to a livestock reservoir. We investigated the molecular epidemiology of the associated MRSA isolates using whole genome sequencing (WGS). Single nucleotide polymorphisms (SNP) were defined and compared to a reference genome to place the isolates into a phylogenetic context. Phylogenetic analysis revealed two distinct farm-specific clusters comprising isolates from the human case and their own livestock, whereas human and animal isolates from the same farm only differed by a small number of SNPs, which supports the likelihood of zoonotic transmission. Further analyses identified a number of genes and mutations that may be associated with host interaction and virulence. This study demonstrates that mecC-MRSA ST130 isolates are capable of transmission between animals and humans, and underscores the potential of WGS in epidemiological investigations and source tracking of bacterial infections. See accompanying article http://dx.doi.org/10.1002/emmm.201302622

引用

页码：509 / 515

页数：7

共 37 条

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