17β-Estradiol Regulation of the mRNA Expression of T-type Calcium Channel Subunits: Role of Estrogen Receptor α and Estrogen Receptor β

被引:34
作者
Bosch, Martha A. [1 ]
Hou, Jingwen [1 ]
Fang, Yuan [1 ]
Kelly, Niartin J. [1 ]
Ronnekleiv, Oline K. [1 ,2 ,3 ]
机构
[1] Oregon Hlth & Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97239 USA
[2] Oregon Hlth & Sci Univ, Dept Anesthesiol & Perioperat Med, Portland, OR 97239 USA
[3] Oregon Natl Primate Res Ctr, Div Neurosci, Beaverton, OR 97005 USA
关键词
Cav3.1; Cav3.2; Cav3.3; alpha; 1; subunits; hypothalamus; pituitary; HORMONE NEURONS; HYPOTHALAMIC NEURONS; INTERNATIONAL-UNION; GENE-EXPRESSION; IONIC CHANNELS; CA2+ CHANNELS; ER-ALPHA; ESTRADIOL; PITUITARY; CURRENTS;
D O I
10.1002/cne.21901
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Low-voltage-activated (T-type) calcium channels are responsible for burst firing and transmitter release in neurons and are important for exocytosis and hormone secretion in pituitary cells. T-type channels contain an alpha 1 subunit, of which there are three subtypes, Cav3.1, -3.2, and -3.3, and each subtype has distinct kinetic characteristics. Although 17 beta-estradiol (E2) modulates T-type calcium channel expression and function, little is known about the molecular mechanisms involved. We used real-time PCR quantification of RNA extracted from hypothalamic nuclei and pituitary in vehicle and E2-treated C57BL/6 mice to elucidate E2-mediated regulation of Cav3.1, -3.2, and -3.3 subunits. The three subunits were expressed in both the hypothalamus and the pituitary. E2 treatment increased the mRNA expression of Cav3.1 and -3.2, but not Cav3.3, in the medial preoptic area and the arcuate nucleus. In the pituitary, Cav3.1 was increased with E2 treatment, and Cav3.2 and -3.3 were decreased. To examine whether the classical estrogen receptors (ERs) were involved in the regulation, we used ER alpha- and ER beta-deficient C57BL/6 mice and explored the effects of E2 on T-type channel subtypes. Indeed, we found that the E2-induced increase in Cav3.1 in the hypothalamus was dependent on ER alpha, whereas the E2 effect on Cav3.2 was dependent on both ERa and ER beta. However, the E2-induced effects in the pituitary were dependent on only the expression of ERa. The robust E2 regulation of T-type calcium channels could be an important mechanism by which E2 increases the excitability of hypothalamic neurons and modulates pituitary secretion. J. Comp. Neurol. 512: 347-358, 2009. (C) 2008 Wiley-Liss, Inc.
引用
收藏
页码:347 / 358
页数:12
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