Gold nanorods reinforced silk fibroin nanocomposite for peripheral nerve tissue engineering applications

被引：31

作者：

Afjeh-Dana, Elham ^{[1
,2
]}

Naserzadeh, Parvaneh ^{[3
,4
]}

Nazari, Hojjatollah ^{[1
,2
]}

Mottaghitalab, Fatemeh ^{[5
]}

Shabani, Ronak ^{[1
,6
]}

Aminii, Naser ^{[1
,7
]}

Mehravi, Bita ^{[1
,2
]}

Rostami, Fatemeh Tajik ^{[8
]}

Joghataei, Mohammad Taghi ^{[1
,6
]}

Mousavizadeh, Kazem ^{[1
,9
]}

Ashtari, Khadijeh ^{[1
,2
]}

机构：

[1] Iran Univ Med Sci, Cellular & Mol Res Ctr, Tehran, Iran

[2] Iran Univ Med Sci, Fac Adv Technol Med, Dept Med Nanotechnol, Tehran 1346855649, Iran

[3] Shehdad Ronak Commercializat Co, SPE, CRN 10320821698, Tehran, Iran

[4] Shahid Beheshti Univ Med Sci, Dept Pharmacol & Toxicol, Fac Pharm, Tehran, Iran

[5] Univ Tehran Med Sci, Nanotechnol Res Ctr, Fac Pharm, Tehran, Iran

[6] Iran Univ Med Sci, Dept Anat, Fac Med, Tehran, Iran

[7] Iran Univ Med Sci, Inst Regenerat Med, Fac Adv Technol Med, Tehran, Iran

[8] Iran Univ Med Sci, Dept Internal Med, Fac Med, Tehran, Iran

[9] Iran Univ Med Sci, Fac Adv Technol Med, Dept Mol Med, Tehran, Iran

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 129卷

关键词：

Peripheral nerve regeneration; Silk fibroin; Gold nanorods; Nanocomposite; CONDUITS; NANOPARTICLES; REGENERATION;

D O I：

10.1016/j.ijbiomac.2019.02.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Nowadays, regenerating peripheral nerves injuries (PNIs) remain a major clinical challenge, which has gained a great attention between scientists. Here, we represent a nanocomposite based on silk fibroin reinforced gold nanorods (SF/GNRs) to evaluate the proliferation and attachment of PC12 cells. The morphological characterization of nanocomposites with transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) showed that the fabricated scaffolds have porous structure with interconnected pores that is suitable for cell adhesion and growth. GNRs significantly improved the poor electrical conductivity of bulk silk fibroin scaffold. Evaluating the morphology of PC12 cells on the scaffold also confirmed the normal morphology of cells with good rate of adhesion. SF/GNRs nanocomposites showed better cellular attachment, growth and proliferation without any toxicity compared with bulk SF scaffold. Moreover, immunostaining studies represented the overexpression of neural specific proteins like nestin and neuron specific enolase (NSE) in the cells cultured on SF/GNRs nanocomposites in comparison to neat SF scaffolds. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：1034 / 1039

页数：6

共 31 条

[1]

End-to-side nerve repair in peripheral nerve injury [J].