Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

被引:67
作者
Indebetouw, Guy [1 ]
Tada, Yoshitaka
Rosen, Joseph
Brooker, Gary
机构
[1] Virginia Tech, Dept Phys, Blacksburg, VA 24061 USA
[2] Johns Hopkins Univ, Integrated Imaging Ctr, Dept Biol, Rockville, MD 20850 USA
[3] Ben Gurion Univ Negev, Dept Elect & Comp Engn, IL-84105 Beer Sheva, Israel
关键词
D O I
10.1364/AO.46.000993
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.
引用
收藏
页码:993 / 1000
页数:8
相关论文
共 52 条