Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates

被引：119

作者：

Agarwal, Paresh ^{[1
]}

Kudirka, Romas ^{[1
]}

Albers, Aaron E. ^{[1
]}

Barfield, Robyn M. ^{[1
]}

de Hart, Gregory W. ^{[1
]}

Drake, Penelope M. ^{[1
]}

Jones, Lesley C. ^{[1
]}

Rabuka, David ^{[1
]}

机构：

[1] Redwood Biosci, Emeryville, CA 94608 USA

来源：

BIOCONJUGATE CHEMISTRY | 2013年 / 24卷 / 06期

基金：

美国国家科学基金会;

关键词：

ACID; ANTIBODY; STABILITY; IMMOBILIZATION; IDENTIFICATION; CHEMISTRY; MECHANISM; OXIME;

D O I：

10.1021/bc400042a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aldehyde- and ketone-functionalized biomolecules have found widespread use in biochemical and biotechnological fields. They are typically conjugated with hydrazide or aminooxy nucleophiles under acidic conditions to yield hydrazone or oxime products that are relatively stable, but susceptible to hydrolysis over time. We introduce a new reaction, the hydrazino-Pictet-Spengler (HIPS) ligation, which has two distinct advantages over hydrazone and oxime ligations. First, the HIPS ligation proceeds quickly near neutral pH, allowing for one-step labeling of aldehyde-functionalized proteins under mild conditions. Second, the HIPS ligation product is very stable (>5 days) in human plasma relative to an oxime-linked conjugate (similar to 4 day), as demonstrated by monitoring protein-fluorophore conjugates by ELISA. Thus, the HIPS ligation exhibits a combination of product stability and speed near neutral pH that is unparalleled by current carbonyl bioconjugation chemistries.

引用

页码：846 / 851

页数：6

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