Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution

被引：89

作者：

Rannes, Julie B. ^{[1
]}

Ioannou, Avgousta ^{[1
]}

Willies, Simon C. ^{[1
]}

Grogan, Gideon ^{[2
]}

Behrens, Carsten ^{[3
]}

Flitsch, Sabine L. ^{[1
]}

Turner, Nicholas J. ^{[1
]}

机构：

[1] Univ Manchester, Sch Chem, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England

[2] Univ York, Dept Chem, York YO10 5YW, N Yorkshire, England

[3] Novo Nordisk AS, Dept Prot & Peptide Chem, Malov, Denmark

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 22期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

ENZYMES; PROTEINS; LACTOSE;

D O I：

10.1021/ja2018477

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.

引用

页码：8436 / 8439

页数：4

共 31 条

[1] OXIDATION RATES OF SOME DESIALYLATED GLYCOPROTEINS BY GALACTOSE-OXIDASE [J].

AVIGAD, G .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 239 (02) :531-537

[2]

Bütler T, 2001, CHEMBIOCHEM, V2, P884

[3] Chemoselective modification of proteins: hitting the target [J].

Carrico, Isaac S. .

CHEMICAL SOCIETY REVIEWS, 2008, 37 (07) :1423-1431

[4] Synthesis and modifications of carbohydrates, using biotransformations [J].

Daines, AM ;

Maltman, BA ;

Flitsch, SL .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2004, 8 (02) :106-113

[5] Enhanced fructose oxidase activity in a galactose oxidase variant [J].

Deacon, SE ;

Mahmoud, K ;

Spooner, RK ;

Firbank, SJ ;

Knowles, PF ;

Phillips, SEV ;

McPherson, MJ .

CHEMBIOCHEM, 2004, 5 (07) :972-979

[6] Exploring and exploiting the therapeutic potential of glycoconjugates [J].

Doores, KJ ;

Gamblin, DP ;

Davis, BG .

CHEMISTRY-A EUROPEAN JOURNAL, 2006, 12 (03) :656-665

[7] Directed evolution of galactose oxidase: Generation of enantioselective secondary alcohol oxidases [J].

Escalettes, Franck ;

Turner, Nicholas J. .

CHEMBIOCHEM, 2008, 9 (06) :857-860

[8] Cofactor processing in galactose oxidase [J].

Firbank, SJ ;

Rogers, M ;

Hurtado-Guerrero, R ;

Dooley, DM ;

Halcrow, MA ;

Phillips, SEV ;

Knowles, PF ;

McPherson, MJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2003, 31 :506-509

[9] A new immunosensing method by galactose oxidase-mediated electrocatalysis using a virtual beaker array [J].

Jeon, Sung Il ;

Hong, Jung Woo ;

Yoon, Hyun C. .

BIOTECHNOLOGY LETTERS, 2006, 28 (17) :1401-1408

[10] Hydrolytic stability of hydrazones and oximes [J].

Kalia, Jeet ;

Raines, Ronald T. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (39) :7523-7526

← 1 2 3 4 →