Directed evolution of galactose oxidase: Generation of enantioselective secondary alcohol oxidases

被引:117
作者
Escalettes, Franck [1 ]
Turner, Nicholas J. [1 ]
机构
[1] Univ Manchester, Sch Chem, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
基金
英国惠康基金;
关键词
alcohol oxidase; biocatalysis; directed evolution; enantioselective catalysis; screening;
D O I
10.1002/cbic.200700689
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
[No abstract available]
引用
收藏
页码:857 / 860
页数:4
相关论文
共 31 条
[1]  
Alexeeva M, 2002, ANGEW CHEM INT EDIT, V41, P3177, DOI 10.1002/1521-3773(20020902)41:17<3177::AID-ANIE3177>3.0.CO
[2]  
2-P
[3]  
ALEXEEVA M, 2002, ANGEW CHEM, V174, P3309
[4]  
Bornscheuer UT, 2006, HYDROLASES IN ORGANIC SYNTHESIS: REGIO- AND STEREOSELECTIVE BIOTRANSFORMATIONS, 2ND EDITION, P1
[5]   Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity [J].
Carr, R ;
Alexeeva, M ;
Enright, A ;
Eve, TSC ;
Dawson, MJ ;
Turner, NJ .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (39) :4807-4810
[6]  
Carr R, 2003, ANGEW CHEM, V115, P4955
[7]   Enhanced fructose oxidase activity in a galactose oxidase variant [J].
Deacon, SE ;
Mahmoud, K ;
Spooner, RK ;
Firbank, SJ ;
Knowles, PF ;
Phillips, SEV ;
McPherson, MJ .
CHEMBIOCHEM, 2004, 5 (07) :972-979
[8]   Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase [J].
Delagrave, S ;
Murphy, DJ ;
Pruss, JLR ;
Maffia, AM ;
Marrs, BL ;
Bylina, EJ ;
Coleman, WJ ;
Grek, CL ;
Dilworth, MR ;
Yang, MM ;
Youvan, DC .
PROTEIN ENGINEERING, 2001, 14 (04) :261-267
[9]   A chemo-enzymatic route to enantiomerically pure cyclic tertiary amines [J].
Dunsmore, CJ ;
Carr, R ;
Fleming, T ;
Turner, NJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (07) :2224-2225
[10]   Stereoinversion of β- and γ-substituted α-amino acids using a chemo-enzymatic oxidation-reduction procedure [J].
Enright, A ;
Alexandre, FR ;
Roff, G ;
Fotheringham, IG ;
Dawson, MJ ;
Turner, NJ .
CHEMICAL COMMUNICATIONS, 2003, (20) :2636-2637