Preferential translation of internal ribosome entry site-containing mRNAs during the mitotic cycle in mammalian cells

被引：136

作者：

Qin, XL ^{[1
]}

Sarnow, P ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2004年 / 279卷 / 14期

关键词：

D O I：

10.1074/jbc.M312854200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cell synchronization protocol was established in which global and individual mRNA translational efficiencies could be examined. While global translational efficiency was reduced in mitotic cells, similar to 3% of mRNAs remained predominantly associated with large polysomes during mitosis, as determined by cDNA microarray analyses. The 5'-non-coding regions of six mRNAs were shown to contain internal ribosome entry sites (IRES). However, not all known mRNAs that contain IRES elements were actively translated during mitosis, arguing that specific IRES sequences are differentially regulated during mitosis.

引用

页码：13721 / 13728

页数：8

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