Electrophoretic manipulation of single DNA molecules in nanofabricated capillaries

被引：71

作者：

Campbell, LC

Wilkinson, MJ

Manz, A

Camilleri, P

Humphreys, CJ

机构：

[1] Univ Cambridge, Dept Mat Sci & Met, Cambridge CB2 3Q2, England

[2] GSK Med Res Ctr, Stevenage SG1 2NY, Herts, England

[3] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AY, England

来源：

LAB ON A CHIP | 2004年 / 4卷 / 03期

关键词：

D O I：

10.1039/b312592k

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the use of nanofabricated capillaries, integrated as part of a microfluidic structure, to study the electrophoretic behaviour of single, fluorescently-labelled, molecules of DNA as a function of capillary size. The nanocapillaries, fabricated using a focused ion beam, have cross-sections down to 150 X 180 nm. Control of single-molecule direction and velocity was achieved using voltage manipulation. DNA mobility was found to increase with decreasing cross-section, which we interpret in terms of reduced electro-osmotic counter-flow. Such nanofabricated capillaries as part of larger fluidic structures have great potential for biotechnology, particularly single molecule manipulation and analysis.

引用

页码：225 / 229

页数：5

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