The Mechanism of Antibacterial Activity of Tetrandrine Against Staphylococcus aureus

被引：29

作者：

Lee, Young-Seob ^{[1
]}

Han, Sin-Hee ^{[1
]}

Lee, Su-Hwan ^{[1
]}

Kim, Young-Guk ^{[1
]}

Park, Chung-Berm ^{[1
]}

Kang, Ok-Hwa ^{[2
,3
]}

Keum, Joon-Ho ^{[2
,3
]}

Kim, Sung-Bae ^{[2
,3
]}

Mun, Su-Hyun ^{[2
,3
]}

Seo, Yun-Soo ^{[2
,3
]}

Myung, Noh-Yil ^{[2
,3
]}

Kwon, Dong-Yeul ^{[2
,3
]}

机构：

[1] Natl Inst Hort & Herbal Sci, Rural Dev Adm, Dept Herbal Crop Res, Eumsung 369873, Chungbuk, South Korea

[2] Wonkang Univ, Coll Pharm, Dept Oriental Pharm, Iksan, Chonbuk, South Korea

[3] Wonkang Univ, Wonkwang Oriental Med Res Inst, Iksan, Chonbuk, South Korea

来源：

FOODBORNE PATHOGENS AND DISEASE | 2012年 / 9卷 / 08期

关键词：

ESCHERICHIA-COLI; IN-VITRO; ETHIDIUM-BROMIDE; MRSA; BACTERIA; EFFLUX; CELLS; EDTA;

D O I：

10.1089/fpd.2011.1119

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Tetrandrine (TET) is a bis-benzylisoquinoline alkaloid derived from the radix of Stephania tetrandra S. Moore. TET performs a wide spectrum of biological activities. The radix of S. tetrandrae has been used traditionally in Asia, including Korea, to treat congestive circulatory disorders and inflammatory diseases. The aim of this study was to examine the mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus. The mechanism was investigated by studying the effects of TET in combination with detergent or membrane potential un-couplers. In addition, the direct involvement of peptidoglycan (PGN) was assessed in titration assays. TET activity against S. aureus was 125-250 mu g/mL, and the minimum inhibitory concentration (MIC) of the two reference strains was 250 mu g/mL. The OD600 of each suspension treated with a combination of ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl) aminomethane (TRIS), and Triton X-100 (TX) with TET (0.25 x MIC) had been reduced from 43% to 96%. Additional structure-function studies on the anti-bacterialactivity of TET in combination with other agents may lead to the discovery of more effective antibacterial agents.

引用

页码：686 / 691

页数：6

共 30 条

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