Analytical and semipreparative high performance liquid chromatography enantio separation of new substituted 1-thiocarbamoyl-3,5-diaryl-4,5-dihydro-(1H)-pyrazoles on polysaccharide-based chiral stationary phases in normal-phase, polar organic and reversed-phase conditions

被引：53

作者：

Cirilli, R

Simonelli, A

Ferretti, R

Bolasco, A

Chimenti, P

Secci, D

Maccioni, E

La Torre, F

机构：

[1] Ist Super Sanita, Dipartimento Farmaco, I-00161 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Studi Chim & Tecnol Sostanze Biol At, I-00185 Rome, Italy

[3] Univ Cagliari, Dipartimento Farmaco Chim Tecnol, I-09124 Cagliari, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1101卷 / 1-2期

关键词：

enantioselective HPLC; polysaccharide-based chiral stationary phases; chiralpak IA; semipreparative scale enantioseparation;

D O I：

10.1016/j.chroma.2005.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The direct HPLC enantioseparation of five pairs of new chiral pyrazole derivatives on coated cellulose- and amylose-based chiral stationary phases (Chiralpak AD, Chiralcel OJ and Chiralcel OJ-RH) and new immobilised amylose-based Chiralpak IA CSP was performed. Very high enantioselectivity factor (a) values were achieved in polar organic and reversed-phase conditions by using OJ-RH as CSP. Chiralpak IA exhibited an excellent chiral resolving ability in normal-phase mode and it allowed the enantioseparation of analytes investigated with resolution factors (Rs)> 20. Due to its bonded nature, it was successfully employed at analytical and semipreparative scale in combination with normal-phase eluents containing "non-standards" solvents such as acetone. (c) 2005 Elsevier B.V. All rights reserved.

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页码：198 / 203

页数：6

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