Analysis of DNA adducts using high-performance separation techniques coupled to electrospray ionization mass spectrometry

被引:76
作者
Andrews, CL
Vouros, P
Harsch, A
机构
[1] Northeastern Univ, Dept Chem, Boston, MA 02115 USA
[2] Northeastern Univ, Barnett Inst, Boston, MA 02115 USA
关键词
mass spectrometry; DNA adducts; nucleosides; nuclleotides; oligonucleotides;
D O I
10.1016/S0021-9673(99)00779-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Identification and quantitation of covalent carcinogen-DNA adducts, an important class of biomarkers, is an integral goal in toxicological research. Since these adducts are commonly present at very low levels in in vivo samples, sensitive and specific analytical methodologies are imperative for accurate detection, characterization and quantitation. High-performance separations coupled to electrospray mass spectrometry (ESI-MS) provide the sensitivity and specificity required for the analysis of DNA adducts. This review provides an overview over the research conducted in this area, focusing on the application of HPLC-ESI-MS and CE-ESI-MS techniques for structural analysis and quantitation of modified nucleosides, nucleotides and oligonucleotides. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:515 / 526
页数:12
相关论文
共 75 条
[1]  
[Anonymous], [No title captured]
[2]   Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry [J].
Apffel, A ;
Chakel, JA ;
Fischer, S ;
Lichtenwalter, K ;
Hancock, WS .
ANALYTICAL CHEMISTRY, 1997, 69 (07) :1320-1325
[3]   Analysis of oligonucleotides by on-column transient capillary isotachophoresis and capillary electrophoresis in poly(ethylene glycol)-filled columns [J].
Auriola, S ;
Jaaskelainen, I ;
Regina, M ;
Urtti, A .
ANALYTICAL CHEMISTRY, 1996, 68 (22) :3907-3911
[4]   Identification of adenine nucleotide-containing metabolites of bisphosphonate drugs using ion-pair liquid chromatography-electrospray mass spectrometry [J].
Auriola, S ;
Frith, J ;
Rogers, MJ ;
Koivuniemi, A ;
Mönkkönen, J .
JOURNAL OF CHROMATOGRAPHY B, 1997, 704 (1-2) :187-195
[5]   Recent advances in capillary electrophoresis electrospray mass spectrometry [J].
Banks, JF .
ELECTROPHORESIS, 1997, 18 (12-13) :2255-2266
[6]   Analysis of modified oligonucleotides by capillary electrophoresis in a polyvinylpyrrolidone matrix coupled with electrospray mass spectrometry [J].
Barry, JP ;
Muth, J ;
Law, SJ ;
Karger, BL ;
Vouros, P .
JOURNAL OF CHROMATOGRAPHY A, 1996, 732 (01) :159-166
[7]   Detection and identification of benzo[a]pyrene diol epoxide adducts to DNA utilizing capillary eleotrophoresis eleotrospray mass spectrometry [J].
Barry, JP ;
Norwood, C ;
Vouros, P .
ANALYTICAL CHEMISTRY, 1996, 68 (08) :1432-1438
[8]   Synthesis, characterization, and quantitation of a 4-aminobiphenyl-DNA adduct standard [J].
Beland, FA ;
Doerge, DR ;
Churchwell, MI ;
Poirier, MC ;
Schoket, B ;
Marques, MM .
CHEMICAL RESEARCH IN TOXICOLOGY, 1999, 12 (01) :68-77
[9]   ANALYSIS OF OLIGONUCLEOTIDES USING COUPLED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY [J].
BLEICHER, K ;
BAYER, E .
CHROMATOGRAPHIA, 1994, 39 (7-8) :405-408
[10]  
CAI J, 1995, J CHROMATOGR, P703