Mitochondria-Targeted Reaction-Based Two-Photon Fluorescent Probe for Imaging of Superoxide Anion in Live Cells and in Vivo

被引：113

作者：

Li, Ping ^{[1
]}

Zhang, Wen ^{[1
]}

Li, Kexiang ^{[1
]}

Liu, Xiao ^{[1
]}

Xiao, Haibin ^{[1
]}

Zhang, Wei ^{[1
]}

Tang, Bo ^{[1
]}

机构：

[1] Shandong Normal Univ, Minist Educ, Key Lab Mol & Nano Probes, Coll Chem Chem Engn & Mat Sci,Engn Res Ctr Pestic, Jinan 250014, Shandong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 20期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

HYDROGEN-PEROXIDE; DESIGN;

D O I：

10.1021/ac402409m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A newly synthesized reaction-based two-photon (TP) fluorescence imaging probe, 9-butyltriphenylphosphoniumacylamino-2,7-dibenzothiazolineflurene (MF-DBZH), composed of a superoxide anion (O-2(center dot-)) responsive group and a mitochondria-targeted site, has been shown to have high selectivity toward mitochondrial O-2(center dot-) fluxes. The fluorescence intensity of MF-DBZH responds proportionally to changes in O-2(center dot-) concentrations. Moreover, MF-DBZH was proved to be insensitive toward pH changes and has high photostability. Favorable features of this probe also include convenient cell loading, easy staining of both cells and small animals, and excellent biocompibility. Most importantly, MF-DBZH gives reliable TP fluorescent signal to changes of O-2(center dot-) levels in vivo.

引用

页码：9877 / 9881

页数：5

共 22 条

[1]

[Anonymous], ANGEW CHEM INT ED

[2] A two-photon fluorescent probe for ratiometric imaging of hydrogen peroxide in live tissue [J].