Imaging of single extended DNA molecules on flat (aminopropyl)triethoxysilane-mica by atomic force microscopy

被引:106
作者
Hu, J
Wang, M
Weier, HUG
Frantz, P
Kolbe, W
Ogletree, DF
Salmeron, M
机构
[1] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV MAT SCI,BERKELEY,CA 94720
[2] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV LIFE SCI,BERKELEY,CA 94720
[3] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,ELECT ENGN DIV,BERKELEY,CA 94720
[4] ACAD SINICA,SHANGHAI INST NUCL RES,SHANGHAI 201800,PEOPLES R CHINA
关键词
D O I
10.1021/la950874c
中图分类号
O6 [化学];
学科分类号
0703 [化学];
摘要
Long strands of DNA have been deposited, extended, and immobilized on chemically modified mica for study with an atomic force microscope (AFM). By employing a modified ''molecular combing'' technique and carefully silanized mica, we show that DNA molecules may be distributed uniformly, adsorbed in extended conformations, and bound with great tenacity. When maintained in a buffer solution, these strands remained stable for > 5 h. Scanning the surface with high loads applied to an AFM tip induced fragmentation in the strands, but remaining pieces never relinquished their original positions. Further imaging applications, such as after treatment of the DNA by molecular hybridization or DNA synthesis and labeling in situ, are discussed.
引用
收藏
页码:1697 / 1700
页数:4
相关论文
共 13 条
[1]
ALIGNMENT AND SENSITIVE DETECTION OF DNA BY A MOVING INTERFACE [J].
BENSIMON, A ;
SIMON, A ;
CHIFFAUDEL, A ;
CROQUETTE, V ;
HESLOT, F ;
BENSIMON, D .
SCIENCE, 1994, 265 (5181) :2096-2098
[2]
ADSORPTION OF DNA TO MICA, SILYLATED MICE, AND MINERALS - CHARACTERIZATION BY ATOMIC-FORCE MICROSCOPY [J].
BEZANILLA, M ;
MANNE, S ;
LANEY, DE ;
LYUBCHENKO, YL ;
HANSMA, HG .
LANGMUIR, 1995, 11 (02) :655-659
[3]
MOTION AND ENZYMATIC DEGRADATION OF DNA IN THE ATOMIC-FORCE MICROSCOPE [J].
BEZANILLA, M ;
DRAKE, B ;
NUDLER, E ;
KASHLEV, M ;
HANSMA, PK ;
HANSMA, HG .
BIOPHYSICAL JOURNAL, 1994, 67 (06) :2454-2459
[4]
BEZANILLA M, 1994, SCANNING MICROSCOPY, V7, P1145
[5]
ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[6]
FOLLOWING THE ASSEMBLY OF RNA-POLYMERASE DNA COMPLEXES IN AQUEOUS-SOLUTIONS WITH THE SCANNING FORCE MICROSCOPE [J].
GUTHOLD, M ;
BEZANILLA, M ;
ERIE, DA ;
JENKINS, B ;
HANSMA, HG ;
BUSTAMANTE, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12927-12931
[7]
ATOMIC FORCE MICROSCOPY OF DNA IN AQUEOUS-SOLUTIONS [J].
HANSMA, HG ;
BEZANILLA, M ;
ZENHAUSERN, F ;
ADRIAN, M ;
SINSHEIMER, RL .
NUCLEIC ACIDS RESEARCH, 1993, 21 (03) :505-512
[8]
TAPPING MODE ATOMIC-FORCE MICROSCOPY IN LIQUIDS [J].
HANSMA, PK ;
CLEVELAND, JP ;
RADMACHER, M ;
WALTERS, DA ;
HILLNER, PE ;
BEZANILLA, M ;
FRITZ, M ;
VIE, D ;
HANSMA, HG ;
PRATER, CB ;
MASSIE, J ;
FUKUNAGA, L ;
GURLEY, J ;
ELINGS, V .
APPLIED PHYSICS LETTERS, 1994, 64 (13) :1738-1740
[9]
ATOMIC FORCE MICROSCOPY IMAGING OF T4 BACTERIOPHAGES ON SILICON SUBSTRATES [J].
KOLBE, WF ;
OGLETREE, DF ;
SALMERON, MB .
ULTRAMICROSCOPY, 1992, 42 :1113-1117
[10]
ATOMIC FORCE MICROSCOPY OF LONG DNA - IMAGING IN AIR AND UNDER WATER [J].
LYUBCHENKO, Y ;
SHLYAKHTENKO, L ;
HARRINGTON, R ;
ODEN, P ;
LINDSAY, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (06) :2137-2140