Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting

被引：18

作者：

Lumeng, CN ^{[1
]}

Hauser, M ^{[1
]}

Brown, V ^{[1
]}

Chamberlain, JS ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Dept Human Genet, Ann Arbor, MI 48109 USA

来源：

BRAIN RESEARCH | 1999年 / 830卷 / 01期

关键词：

dystrophin; Dp71; knockout mice; reporter gene;

D O I：

10.1016/S0006-8993(99)01201-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To investigate the function of the major non-muscle dystrophin isoform, Dp71. we substituted a beta-galactosidase (beta gal) reporter gene for Dp71 by homologous recombination in embryonic stem cells. Staining for beta gal activity in chimeric mice revealed Dp71 promoter activity in glial cells in the CNS, in neurons of the inner nuclear and inner plexiform layers of the retina, and in the kidney tubules and collecting ducts. Our observations demonstrate that Dp71 is widely expressed in the adult CNS (retina, cerebellum, cerebral cortex, ependyma, and choroid) as well as the adult kidney epithelium and suggest a broad function for Dp71 in differentiated tissues. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：174 / 178

页数：5

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