Visualization of the degradation of a disulfide polymer, linear poly(ethylenimine sulfide), for gene delivery

被引：175

作者：

Lee, Yan ^{[1
]}

Mo, Heejung ^{[1
]}

Koo, Heebeom ^{[1
]}

Park, Jong-Yeun ^{[1
]}

Cho, Min Yi ^{[1
]}

Jin, Geun-woo ^{[1
]}

Park, Jong-Sang ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

来源：

BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 01期

关键词：

D O I：

10.1021/bc060113t

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyethylenimine (PEI) shows high transfection efficiency and cytoxicity due to its high amine density. The new disulfide cationic polymer, linear poly(ethylenimine sulfide) (l-PEIS), was synthesized for efficient and safe gene delivery. As the amine density of l-PEIS increased, the transfection efficiency also increased. l-PEIS-6 and l-PEIS-8 show transfection efficiencies that are similar to that of PEI. However, cytotoxicity of l-PEIS was not observed due to the biodegradable disulfide bond. The disulfide bonds are stable in the oxidative extracellular condition and can be degraded rapidly in the reductive intracellular condition. The degradation of l-PEIS in HeLa cells was visualized by fluorescence microscopy using the probe-probe dequenching effect of BODIPY-FL fluorescence dye. l-PEIS was degraded completely within 3 h.

引用

页码：13 / 18

页数：6

共 26 条

[1] Polymeric carriers for oral uptake of microparticulates [J].

Andrianov, AK ;

Payne, LG .

ADVANCED DRUG DELIVERY REVIEWS, 1998, 34 (2-3) :155-170

[2] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[3] Copolymers of ethylene imine and N-(2-hydroxyethyl)-ethylene imine as tools to study effects of polymer structure on physicochemical and biological properties of DNA complexes [J].

Fischer, D ;

von Harpe, A ;

Kunath, K ;

Petersen, H ;

Li, YX ;

Kissel, T .

BIOCONJUGATE CHEMISTRY, 2002, 13 (05) :1124-1133

[4] Poly(ethylenimine) and its role in gene delivery [J].

Godbey, WT ;

Wu, KK ;

Mikos, AG .

JOURNAL OF CONTROLLED RELEASE, 1999, 60 (2-3) :149-160

[5] Chemistry - One generation at a time [J].

Gorman, C .

NATURE, 2002, 415 (6871) :487-488

[6] Efficient gene transfer using reversibly cross-linked low molecular weight polyethylenimine [J].

Gosselin, MA ;

Guo, WJ ;

Lee, RJ .

BIOCONJUGATE CHEMISTRY, 2001, 12 (06) :989-994

[7]

HAUGLAND RP, 2000, Patent No. 5719031

[8] Poly(ortho esters): synthesis, characterization, properties and uses [J].

Heller, J ;

Barr, J ;

Ng, SY ;

Abdellauoi, KS ;

Gurny, R .

ADVANCED DRUG DELIVERY REVIEWS, 2002, 54 (07) :1015-1039

[9]

Hermanson GT, 2013, BIOCONJUGATE TECHNIQUES, 3RD EDITION, P341, DOI 10.1016/B978-0-12-382239-0.00007-8

[10] Mechanism of cell transfection with plasmid/chitosan complexes [J].

Ishii, T ;

Okahata, Y ;

Sato, T .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2001, 1514 (01) :51-64

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