Preliminary study on Emodin alleviating alpha-naphthylisothiocyanate-induced intrahepatic cholestasis by regulation of liver farnesoid X receptor pathway

被引：29

作者：

Ding, Yan ^{[1
]}

Xiong, Xiao-Li ^{[2
]}

Zhou, Li-Shan ^{[2
]}

Yan, Su-Qi ^{[2
]}

Qin, Huan ^{[3
]}

Li, Hua-Rong ^{[4
]}

Zhang, Ling-Ling ^{[2
]}

Chen, Peng ^{[5
]}

Yao, Cong ^{[6
]}

Jiang, Zhi-Xia ^{[2
]}

Zhao, Lei ^{[7
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Infect Dis & Immunol, Wuhan Childrens Hosp,Tongji Med Coll, Wuhan Med & Healthcare Ctr Women & Children, Wuhan, Peoples R China

[2] Huazhong Univ Sci & Technol, Dept Integrated Chinese & Western Med, Wuhan Childrens Hosp,Tongji Med Coll, Wuhan Med & Healthcare Ctr Women & Children, Wuhan, Peoples R China

[3] Huazhong Univ Sci & Technol, Dept Clin Lab, Wuhan Med & Healthcare Ctr Women & Children, Wuhan Childrens Hosp,Tongji Med Coll, Wuhan, Peoples R China

[4] Huazhong Univ Sci & Technol, Dept Integrated Chinese & Western Med, Union Hosp, Tongji Med Coll, Wuhan, Peoples R China

[5] Huazhong Univ Sci & Technol, Wuhan Childrens Hosp, Tongji Med Coll,Dept Respirat, Wuhan Med & Healthcare Ctr Women & Children, Wuhan, Peoples R China

[6] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp,Dept Hlth, Wuhan Med & Healthcare Ctr Women & Children, Wuhan, Peoples R China

[7] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Infect Dis, Wuhan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY | 2016年 / 29卷 / 04期

基金：

美国国家科学基金会;

关键词：

alpha-naphthyl isothiocyanate; Emodin; farnesoid X receptor (FXR); intrahepatic cholestasis; CORILAGIN; FIBROSIS; ACID; FXR; EXPLORATION; TLR2;

D O I：

10.1177/0394632016672218

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

The aim of this study is to investigate Emodin on alleviating intrahepatic cholestasis by regulation of liver farnesoid X receptor (FXR) pathway. Cell and animal models of intrahepatic cholestatis were established. Biochemical tests and histomorphology were performed. The messenger RNA (mRNA) and protein expression of FXR, small heterodimer partner (SHP), uridine diphosphate glucuronosyltransferase 2 family polypeptide B4 (UGT2B4), and bile salt export pump (BSEP) was detected. As a result, compared with the model group, the serum levels of biochemical test were significantly lower in the Emodin group (P < 0.01). The histopathological changes were remitted significantly by Emodin treatment. In the model group, the mRNA and protein expression of FXR, SHP, UGT2B4, and BSEP was significantly lower than in the normal group in cell models (P < 0.05). With Emodin intervention, the expression of FXR, SHP, UGT2B4, and BSEP was notably increased (P < 0.05). In conclusion, Emodin plays a protective role in intrahepatic cholestasis by promoting FXR signal pathways.

引用

页码：805 / 811

页数：7

共 18 条

[1]

Beyond Bile Acids: Targeting Farnesoid X Receptor (FXR) with Natural and Synthetic Ligands [J].