Assessment of the genotoxicity of contaminated soil with the Allium/Vicia-micronucleus and the Tradescantia-micronucleus assays

被引:91
作者
Cotelle, S [1 ]
Masfaraud, JF [1 ]
Férard, JF [1 ]
机构
[1] Univ Metz, Ctr Environm Sci, F-57040 Metz 1, France
关键词
genotoxicity; micronucleus assay; contaminated soil; Allium cepa; Vicia faba; Tradescantia;
D O I
10.1016/S0027-5107(99)00063-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The present study concerns the genotoxicity of contaminated soil near Metz, France. Three plant bioassays, the Vicia faba (broad bean), the Allium cepa (white onion) and the Tradescnntia (spiderwort) micronuclei tests were used to evaluate for genotoxicity. Two soil samples were tested: soil sample A (from an industrial waste site) and soil sample B (from a cokeworks waste site). Maleic hydrazide was used as the positive control. Aqueous extracts of the soil samples were used to treat the roots of Vicia and Allium, and plant cuttings of Tradescantia according to the standard protocol for these plant assays established by the International Program on Chemical Safety and the World Health Organization. The results of these tests showed differential sensitivity in the three different bioassays. Soil sample A was more toxic than soil sample B. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:167 / 171
页数:5
相关论文
共 15 条
[1]   REDUCTION OF GENOTOXICITY OF A CREOSOTE-CONTAMINATED SOIL AFTER FUNGAL TREATMENT DETERMINED BY THE TRADESCANTIA-MICRONUCLEUS TEST [J].
BAUDGRASSET, S ;
BAUDGRASSET, F ;
BIFULCO, JM ;
MEIER, JR ;
MA, TH .
MUTATION RESEARCH, 1993, 303 (02) :77-82
[2]   CHROMOSOME ABERRATION ASSAYS IN ALLIUM [J].
GRANT, WF .
MUTATION RESEARCH, 1982, 99 (03) :273-291
[3]  
Hoagland D.R., 1950, CALIF AES C, P1, DOI DOI 10.1007/S12374-010-9112-0
[4]   VICIA-FABA CHROMOSOMAL ABERRATION ASSAY [J].
KANAYA, N ;
GILL, BS ;
GROVER, IS ;
MURIN, A ;
OSIECKA, R ;
SANDHU, SS ;
ANDERSSON, HC .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1994, 310 (02) :231-247
[5]   THE IMPROVED ALLIUM VICIA ROOT-TIP MICRONUCLEUS ASSAY FOR CLASTOGENICITY OF ENVIRONMENTAL-POLLUTANTS [J].
MA, TH ;
XU, ZD ;
XU, CG ;
MCCONNELL, H ;
RABAGO, EV ;
ARREOLA, GA ;
ZHANG, HG .
MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS, 1995, 334 (02) :185-195
[6]   In situ monitoring with the Tradescantia bioassays on the genotoxicity of gaseous emissions from a closed landfill site and an incinerator [J].
Ma, TH ;
Xu, C ;
Liao, S ;
McConnell, H ;
Jeong, BS ;
Won, CD .
MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS, 1996, 359 (01) :39-52
[7]   TRADESCANTIA-MICRONUCLEUS BIOASSAY [J].
MA, TH ;
CABRERA, GL ;
CHEN, R ;
GILL, BS ;
SANDHU, SS ;
VANDENBERG, AL ;
SALAMONE, MF .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1994, 310 (02) :221-230
[8]  
MONARCA S, IN PRESS MUTATION S
[9]   Persistence of cadmium-induced adaptive response to genotoxicity of maleic hydrazide and methyl mercuric chloride in root meristem cells of Allium cepa L: Differential inhibition by cycloheximide and buthionine sulfoximine [J].
Panda, KK ;
Patra, J ;
Panda, BB .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1997, 389 (2-3) :129-139
[10]   MONITORING AND ASSESSMENT OF MERCURY POLLUTION IN THE VICINITY OF A CHLORALKALI PLANT .3. CONCENTRATION AND GENOTOXICITY OF MERCURY IN THE INDUSTRIAL EFFLUENT AND CONTAMINATED WATER OF RUSHIKULYA ESTUARY, INDIA [J].
PANDA, KK ;
LENKA, M ;
PANDA, BB .
MUTATION RESEARCH, 1992, 280 (03) :149-160