A simple and rapid method for evaluation of Mark-Houwink-Sakurada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusion chromatography: application to guar galactomannan

被引：77

作者：

Beer, MU ^{[1
]}

Wood, PJ

Weisz, J

机构：

[1] Novartis Nutr Res AG, CH-3176 Neuenegg, Switzerland

[2] Univ Guelph, Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, Guelph, ON N1G 2W1, Canada

来源：

CARBOHYDRATE POLYMERS | 1999年 / 39卷 / 04期

关键词：

high performance size exclusion chromatography; polysaccharide; guar galactomannan; partial acid hydrolysis;

D O I：

10.1016/S0144-8617(99)00017-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A rapid method for establishing the constants in the Mark-Houwink-Sakurada equation, relating intrinsic viscosity and molecular weight (MW), of guar galactomannan is described. Following partial acid hydrolysis, the galactomannan was analyzed using high performance size exclusion chromatography employing viscosimetry and right angle light scattering detectors. In this way, a large number of samples of polysaccharides with a wide range of MW distributions were prepared, without need for isolation, and intrinsic viscosity and MW rapidly determined. The a and K values found for guar galactomannan were 0.72 and 5.13 x 10(-4) ([eta] in dl/g) respectively, in good agreement with previously published values. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：377 / 380

页数：4

共 12 条

[1] Wheat bread supplemented with depolymerized guar gum reduces the plasma cholesterol concentration in hypercholesterolemic human subjects [J].