A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

被引：55

作者：

Ma, Ming ^{[1
]}

Ye, Adam Y. ^{[2
,3
]}

Zheng, Weiguo ^{[4
]}

Kong, Lei ^{[2
]}

机构：

[1] Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China

[2] Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Ctr Bioinformat, Beijing 100871, Peoples R China

[3] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[4] Peking Univ, Inst Comp Sci & Technol, Beijing 100871, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2013年 / 2013卷

关键词：

NUCLEASE; GENERATION; CELLS;

D O I：

10.1155/2013/270805

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.

引用

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