A local regulatory network around three NAC transcription factors in stress responses and senescence in Arabidopsis leaves

被引：189

作者：

Hickman, Richard ^{[1
]}

Hill, Claire ^{[2
]}

Penfold, Christopher A. ^{[1
]}

Breeze, Emily ^{[1
,2
]}

Bowden, Laura ^{[2
]}

Moore, Jonathan D. ^{[1
]}

Zhang, Peijun ^{[2
]}

Jackson, Alison ^{[2
]}

Cooke, Emma ^{[3
]}

Bewicke-Copley, Findlay ^{[2
]}

Mead, Andrew ^{[2
]}

Beynon, Jim ^{[1
,2
]}

Wild, David L. ^{[1
]}

Denby, Katherine J. ^{[1
,2
]}

Ott, Sascha ^{[1
]}

Buchanan-Wollaston, Vicky ^{[1
,2
]}

机构：

[1] Univ Warwick, Warwick Syst Biol Ctr, Coventry CV4 7AL, W Midlands, England

[2] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England

[3] Univ Warwick, Mol Org & Assembly Cells Doctoral Training Ctr, Coventry CV4 7AL, W Midlands, England

来源：

PLANT JOURNAL | 2013年 / 75卷 / 01期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; Botrytis cinerea; NAC transcription factors; gene regulatory network; senescence; stress; DNA-BINDING SPECIFICITY; GENE-EXPRESSION; LOW-TEMPERATURE; ABSCISIC-ACID; SALT TOLERANCE; DROUGHT; FAMILY; ACTIVATION; MICROARRAY; PROTEINS;

D O I：

10.1111/tpj.12194

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A model is presented describing the gene regulatory network surrounding three similar NAC transcription factors that have roles in Arabidopsis leaf senescence and stress responses. ANAC019, ANAC055 and ANAC072 belong to the same clade of NAC domain genes and have overlapping expression patterns. A combination of promoter DNA/protein interactions identified using yeast 1-hybrid analysis and modelling using gene expression time course data has been applied to predict the regulatory network upstream of these genes. Similarities and divergence in regulation during a variety of stress responses are predicted by different combinations of upstream transcription factors binding and also by the modelling. Mutant analysis with potential upstream genes was used to test and confirm some of the predicted interactions. Gene expression analysis in mutants of ANAC019 and ANAC055 at different times during leaf senescence has revealed a distinctly different role for each of these genes. Yeast 1-hybrid analysis is shown to be a valuable tool that can distinguish clades of binding proteins and be used to test and quantify protein binding to predicted promoter motifs.

引用

页码：26 / 39

页数：14

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