A local regulatory network around three NAC transcription factors in stress responses and senescence in Arabidopsis leaves

被引:189
作者
Hickman, Richard [1 ]
Hill, Claire [2 ]
Penfold, Christopher A. [1 ]
Breeze, Emily [1 ,2 ]
Bowden, Laura [2 ]
Moore, Jonathan D. [1 ]
Zhang, Peijun [2 ]
Jackson, Alison [2 ]
Cooke, Emma [3 ]
Bewicke-Copley, Findlay [2 ]
Mead, Andrew [2 ]
Beynon, Jim [1 ,2 ]
Wild, David L. [1 ]
Denby, Katherine J. [1 ,2 ]
Ott, Sascha [1 ]
Buchanan-Wollaston, Vicky [1 ,2 ]
机构
[1] Univ Warwick, Warwick Syst Biol Ctr, Coventry CV4 7AL, W Midlands, England
[2] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England
[3] Univ Warwick, Mol Org & Assembly Cells Doctoral Training Ctr, Coventry CV4 7AL, W Midlands, England
基金
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
Arabidopsis thaliana; Botrytis cinerea; NAC transcription factors; gene regulatory network; senescence; stress; DNA-BINDING SPECIFICITY; GENE-EXPRESSION; LOW-TEMPERATURE; ABSCISIC-ACID; SALT TOLERANCE; DROUGHT; FAMILY; ACTIVATION; MICROARRAY; PROTEINS;
D O I
10.1111/tpj.12194
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A model is presented describing the gene regulatory network surrounding three similar NAC transcription factors that have roles in Arabidopsis leaf senescence and stress responses. ANAC019, ANAC055 and ANAC072 belong to the same clade of NAC domain genes and have overlapping expression patterns. A combination of promoter DNA/protein interactions identified using yeast 1-hybrid analysis and modelling using gene expression time course data has been applied to predict the regulatory network upstream of these genes. Similarities and divergence in regulation during a variety of stress responses are predicted by different combinations of upstream transcription factors binding and also by the modelling. Mutant analysis with potential upstream genes was used to test and confirm some of the predicted interactions. Gene expression analysis in mutants of ANAC019 and ANAC055 at different times during leaf senescence has revealed a distinctly different role for each of these genes. Yeast 1-hybrid analysis is shown to be a valuable tool that can distinguish clades of binding proteins and be used to test and quantify protein binding to predicted promoter motifs.
引用
收藏
页码:26 / 39
页数:14
相关论文
共 60 条
[41]   A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation [J].
Ou, Bin ;
Yin, Kang-Quan ;
Liu, Sai-Nan ;
Yang, Yan ;
Gu, Tren ;
Hui, Jennifer Man Wing ;
Zhang, Li ;
Miao, Jin ;
Kondou, Youichi ;
Matsui, Minami ;
Gu, Hong-Ya ;
Qu, Li-Jia .
MOLECULAR PLANT, 2011, 4 (03) :546-555
[42]   Isolation and functional characterization of the Arabidopsis salt-tolerance 32 (AtSAT32) gene associated with salt tolerance and ABA signaling [J].
Park, Min-Young ;
Chung, Moon-Soo ;
Koh, Hee-Seok ;
Lee, Dong J. ;
Ahn, Sung-Ju ;
Kim, Cheol S. .
PHYSIOLOGIA PLANTARUM, 2009, 135 (04) :426-435
[43]   Nonparametric Bayesian inference for perturbed and orthologous gene regulatory networks [J].
Penfold, Christopher A. ;
Buchanan-Wollaston, Vicky ;
Denby, Katherine J. ;
Wild, David L. .
BIOINFORMATICS, 2012, 28 (12) :I233-I241
[44]   How to infer gene networks from expression profiles, revisited [J].
Penfold, Christopher A. ;
Wild, David L. .
INTERFACE FOCUS, 2011, 1 (06) :857-870
[45]   A Functional Genomics Approach Reveals CHE as a Component of the Arabidopsis Circadian Clock [J].
Pruneda-Paz, Jose L. ;
Breton, Ghislain ;
Para, Alessia ;
Kay, Steve A. .
SCIENCE, 2009, 323 (5920) :1481-1485
[46]   NAC proteins: regulation and role in stress tolerance [J].
Puranik, Swati ;
Sahu, Pranav Pankaj ;
Srivastava, Prem S. ;
Prasad, Manoj .
TRENDS IN PLANT SCIENCE, 2012, 17 (06) :369-381
[47]   Arabidopsis transcription factors:: Genome-wide comparative analysis among eukaryotes [J].
Riechmann, JL ;
Heard, J ;
Martin, G ;
Reuber, L ;
Jiang, CZ ;
Keddie, J ;
Adam, L ;
Pineda, O ;
Ratcliffe, OJ ;
Samaha, RR ;
Creelman, R ;
Pilgrim, M ;
Broun, P ;
Zhang, JZ ;
Ghandehari, D ;
Sherman, BK ;
Yu, CL .
SCIENCE, 2000, 290 (5499) :2105-2110
[48]   DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression [J].
Sakuma, Y ;
Liu, Q ;
Dubouzet, JG ;
Abe, H ;
Shinozaki, K ;
Yamaguchi-Shinozaki, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 290 (03) :998-1009
[49]   CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis [J].
Schuhegger, Regina ;
Nafisi, Majse ;
Mansourova, Madina ;
Petersen, Bent Larsen ;
Olsen, Carl Erik ;
Svatos, Ales ;
Halkier, Barbara Ann ;
Glawischnig, Erich .
PLANT PHYSIOLOGY, 2006, 141 (04) :1248-1254
[50]   DUAL DNA-BINDING SPECIFICITY OF A PETAL EPIDERMIS-SPECIFIC MYB TRANSCRIPTION FACTOR (MYB.PH3) FROM PETUNIA-HYBRIDA [J].
SOLANO, R ;
NIETO, C ;
AVILA, J ;
CANAS, L ;
DIAZ, I ;
PAZARES, J .
EMBO JOURNAL, 1995, 14 (08) :1773-1784