Gas-pressure-driven stepwise-gradient elution system for microcolumn liquid chromatography

被引：3

作者：

Takeuchi, T ^{[1
]}

Lim, LW ^{[1
]}

机构：

[1] Gifu Univ, Fac Engn, Dept Chem, Gifu 5011193, Japan

来源：

BUNSEKI KAGAKU | 2001年 / 50卷 / 12期

关键词：

gas-pressure-driven flow; stepwise-gradient elution; postcolumn-controlled flow system; microcolumn liquid chromatography;

D O I：

10.2116/bunsekikagaku.50.825

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Stepwise-gradient elution was carried out with a gas-pressure-driven flow system. The system was assembled from a gas cylinder, a 6-way selection valve, an injection valve (or a sample-enrichment system), a separation column, a UV detector and a syringe pump. Eluents with different compositions were supplied by pressure applied from an argon gas cylinder; switching the 6-way selection valve changed the eluent composition. The syringe pump located downstream of the detector regulated the flow rate of the eluent. The relative standard deviation of the retention time was 0.4-0.6% for isocratic elution as well as for two-step gradient elution. The system was applied to die separation of phthalates and alkylbenzenes.

引用

页码：825 / 828

页数：4

共 12 条

[1] Evaluation of automated isocratic and gradient nano-liquid chromatography and capillary electrochromatography [J].