Quantum dot-encoded mesoporous beads with high brightness and uniformity: Rapid readout using flow cytometry

被引:249
作者
Gao, XH
Nie, SM
机构
[1] Emory Univ, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30322 USA
[2] Georgia Inst Technol, Atlanta, GA 30322 USA
[3] Emory Univ, Dept Chem, Atlanta, GA 30322 USA
[4] Emory Univ, Dept Hematol, Atlanta, GA 30322 USA
[5] Emory Univ, Dept Oncol, Atlanta, GA 30322 USA
[6] Emory Univ, Winship Canc Inst, Atlanta, GA 30322 USA
关键词
D O I
10.1021/ac0354600
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new generation of optically encoded beads has been prepared by using mesoporous polystyrene beads and surfactant-coated semiconductor quantum dots. In comparison with nonporous beads of similar sizes and chemical compositions, the encoded porous beads are similar to 1000 times brighter and 5 times more uniform in fluorescence intensities. Using both absolute intensity and ratiometric fluorescence coding, we show that the beads can be identified with a standard flow cytometer at 1000 beads/s. This result indicates that the multiple excited-state lifetimes and relaxation pathways of quantum dots do not limit their applications in high-speed optical detection and imaging.
引用
收藏
页码:2406 / 2410
页数:5
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