Time-resolved fluorescence correlation spectroscopy

被引：132

作者：

Böhmer, M

Wahl, M

Rahn, HJ

Erdmann, R

Enderlein, J ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biol Informat Proc 1, D-52425 Julich, Germany

[2] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany

[3] PicoQunt GmbH, D-12489 Berlin, Germany

来源：

CHEMICAL PHYSICS LETTERS | 2002年 / 353卷 / 5-6期

关键词：

D O I：

10.1016/S0009-2614(02)00044-1

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A new method of performing fluorescence correlation spectroscopy (FCS) measurements for mixtures of several fluorescent molecular species is introduced. It uses time-resolved fluorescence detection for separating the different FCS-contributions from the different species. This allows simultaneous and independent monitoring of the diffusion of several molecular species in one sample, or performing multi-label cross-correlation measurements. In this way, the proposed method is equivalent to dual- or multi-color FCS. However, it is simpler to implement experimentally, because it requires only single wavelength excitation and detection. This Letter outlines the theoretical basis and presents experimental results of the method. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：439 / 445

页数：7

共 31 条

[21] Fluorescence cross-correlation:: A new concept for polymerase chain reaction [J].