Renewable microcolumns for solid-phase nucleic acid separations and analysis from environmental samples

被引：36

作者：

Chandler, DP

Brockman, FJ

Holman, DA

Grate, JW

Bruckner-Lea, CJ

机构：

[1] Pacific NW Lab, Environm Microbiol Grp, Richland, WA 99352 USA

[2] Pacific NW Lab, Chem Sensors & Microanalyt Syst, Richland, WA 99352 USA

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2000年 / 19卷 / 05期

基金：

美国能源部;

关键词：

flow injection; sequential injection; DNA; renewable microcolumn; sample preparation; super-paramagnetic bead; microparticle;

D O I：

10.1016/S0165-9936(99)00213-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article summarizes the convergence of sequential injection-renewable separation columns (SI-RSC) and techniques with nucleic acid analysis at the Pacific Northwest National Laboratory. New renewable microcolumn designs, functional principles and their analytical and practical utility are presented within the context of automated nucleic acid extraction and purification from environmental samples. (C) 2000 Published by Elsevier Science B.V.

引用

页码：314 / 321

页数：8

共 23 条

[1] Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples [J].