Purification of Aspergillus sp xylanase by precipitation with an anionic polymer Eudragit S100

被引：21

作者：

Gawande, PV ^{[1
]}

Kamat, MY ^{[1
]}

机构：

[1] Univ Bombay, Dept Chem Technol, Food & Fermentat Technol Div, Bombay 400019, Maharashtra, India

来源：

PROCESS BIOCHEMISTRY | 1999年 / 34卷 / 6-7期

关键词：

Aspergillus sp; xylanase; enzyme purification; affinity precipitation; anionic polymer; Eudragit S100;

D O I：

10.1016/S0032-9592(98)00123-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of xylanases from the crude culture filtrates of Aspergillus sp 5 and Aspergillus sp 44 was carried out using affinity precipitation with a commercially available enteric polymer Eudragit S100. With affinity precipitation the yield of enzyme was 85.3, 82.7% with 10.8, 4.08-folds (specific activity of ammonium sulphate precipitate was taken as 100%) increases in the specific activity of Aspergillus sp 5 and Aspergillus sp 44, respectively. A comparison of SDS-PAGE electrophoretic patterns of the ammonium sulphate and purified enzyme by affinity precipitation showed significant purification of the enzyme. Zymogram analysis revealed recovery of three and two forms of xylanases from Aspergillus sp 5 and Aspergillus sp 44, respectively. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：577 / 580

页数：4

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