The machinery for cell polarity, cell morphogenesis, and the cytoskeleton in the Basidiomycete fungus Ustilago maydis -: A survey of the genome sequence

被引:21
作者
Banuett, Flora [1 ]
Quintanilla, Rene H., Jr. [1 ]
Reynaga-Pena, Cristina G. [2 ]
机构
[1] Calif State Univ Long Beach, Dept Biol Sci, Long Beach, CA 90840 USA
[2] Ctr Invest & Estudios Avanzados IPN, Dept Ingn Genet, Unidad Irapuato, Guanajuato 36821, CP, Mexico
关键词
polarized growth; cytoskeleton; polarisome; fungal morphogenesis; Ustilgo maydis;
D O I
10.1016/j.fgb.2008.05.012
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Ustilago maydis, a Basidiomycete fungus that infects maize, exhibits two basic morphologies, a yeast-like and a filamentous form. The yeast-like cell is elongated, divides by budding, and the bud grows by tip extension. The filamentous form divides at the apical cell and grows by tip extension. The repertoire of morphologies is increased during interaction with its host, suggesting that plant signals play an important role in generation of additional morphologies. We have used Saccharomyces cerevisiae and Schizosaccharomyces pombe genes known to play a role in cell polarity and morphogenesis, and in the cytoskeleton as probes to survey the U. maydis genome. We have found that most of the yeast machinery is conserved in U. maydis, albeit the degree of similarity varies from strong to weak. The U. maydis genome contains the machinery for recognition and interpretation of the budding yeast axial and bipolar landmarks; however, genes coding for some of the landmark proteins are absent. Genes coding for cell polarity establishment, exocytosis, actin and microtubule organization, microtubule plus-end associated proteins, kinesins, and myosins are also present. Genes not present in S. cerevisiae and S. pombe include a homolog of mammalian Rac, a hybrid myosin-chitin synthase, and several kinesins that exhibit more similarity to their mammalian counterparts. We also used the U. maydis genes identified in this analysis to search other fungal and other eukaryotic genomes to identify the closest homologs. In most cases, not surprisingly, the closest homolog is among filamentous fungi, not the yeasts, and in some cases it is among mammals. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:S3 / S14
页数:12
相关论文
共 115 条
[51]   The role of the kinesin motor KipA in microtubule organization and polarized growth of Aspergillus nidulans [J].
Konzack, S ;
Rischitor, PE ;
Enke, C ;
Fischer, R .
MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (02) :497-506
[52]   The cell wall:: a carbohydrate armour for the fungal cell [J].
Latge, Jean-Paul .
MOLECULAR MICROBIOLOGY, 2007, 66 (02) :279-290
[53]   Null mutants of the Neurospora actin-related protein 1 pointed-end complex show distinct phenotypes [J].
Lee, IH ;
Kumar, S ;
Plamann, M .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (07) :2195-2206
[54]   The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast [J].
Lee, WL ;
Oberle, JR ;
Cooper, JA .
JOURNAL OF CELL BIOLOGY, 2003, 160 (03) :355-364
[55]   Characterisation of Aspergillus nidulans polarisome component BemA [J].
Leeder, Abigail C. ;
Turner, Geoffrey .
FUNGAL GENETICS AND BIOLOGY, 2008, 45 (06) :897-911
[56]   A dynein loading zone for retrograde endosome motility at microtubule plus-ends [J].
Lenz, J. H. ;
Schuchardt, I. ;
Straube, A. ;
Steinberg, G. .
EMBO JOURNAL, 2006, 25 (11) :2275-2286
[57]   The PAK family kinase Cla4 is required for budding and morphogenesis in Ustilago maydis [J].
Leveleki, L ;
Mahlert, M ;
Sandrock, B ;
Bölker, M .
MOLECULAR MICROBIOLOGY, 2004, 54 (02) :396-406
[58]   Regulation of septin organization and function in yeast [J].
Longtine, MS ;
Bi, EF .
TRENDS IN CELL BIOLOGY, 2003, 13 (08) :403-409
[59]   Diversity and dynamics of the Spitzenkorper in growing hyphal tips of higher fungi [J].
LopezFranco, R ;
Bracker, CE .
PROTOPLASMA, 1996, 195 (1-4) :90-111
[60]   Rac1 and Cdc42 regulate hyphal growth and cytokinesis in the dimorphic fungus Ustilago maydis [J].
Mahlert, M ;
Leveleki, L ;
Hlubek, A ;
Sandrock, B ;
Bölker, M .
MOLECULAR MICROBIOLOGY, 2006, 59 (02) :567-578