Amperometric sensor based on ferrocene-modified multiwalled carbon nanotube nanocomposites as electron mediator for the determination of glucose

被引：161

作者：

Qiu, Jian-Ding ^{[1
,2
,3
]}

Zhou, Wen-Mei ^{[1
,2
]}

Guo, Jin ^{[1
,2
]}

Wang, Rui ^{[1
,2
]}

Liang, Ru-Ping ^{[1
,2
]}

机构：

[1] Nanchang Univ, Dept Chem, Nanchang 330031, Peoples R China

[2] Nanchang Univ, Inst Adv Study, Nanchang 330031, Peoples R China

[3] Pingxiang Coll, Dept Chem Engn, Pingxiang 337055, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 385卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Nanocomposites; Multiwalled carbon nanotubes; Ferrocenecarboxaldehyde; Chitosan; Glucose oxidase; Biosensor; LAYERED DOUBLE HYDROXIDES; ENZYME-BASED BIOSENSORS; ASCORBIC-ACID; SILICA NANOPARTICLES; OXIDASE; IMMOBILIZATION; COMPOSITE; DOPAMINE; POLYMER; HYBRIDIZATION;

D O I：

10.1016/j.ab.2008.12.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A kind of nanocomposite with good dispersion in water was prepared through covalent adsorption of ferrocenecarboxaldehyde on multiwalled carbon nanotubes (MWNTs) for electrical communication between glucose oxidase (GOD) and electrode. the ferrocene-modified multiwalled carbon nanotube nanocomposites (MWNTs-Fc) Could be conveniently cast on electrode surfaces. With the aid of chitosan, GOD was then immobilized on the nanostructure film to form a reagentless amperometric sensor for glucose determination. FTIR spectra and cyclic voltammetry were used to characterize the nanocomposites. The presence of both ferrocene as mediator of electron transfer and MWNTs as conductor enhanced greatly the enzymatic response to the oxidation of glucose. The novel biosensor exhibited a fast response toward glucose with a detection limit of 3.0 x 10(-6) mol/L and the linear range extended up to 3.8 x 10(-3) mol/L. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：264 / 269

页数：6

共 54 条

[21] Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor [J].

Li, J ;

Wang, YB ;

Qiu, JD ;

Sun, D ;

Xia, XH .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 383 (06) :918-922

[22] Electrochemical genosensing properties of gold nanoparticle-carbon nanotube hybrid [J].

Lim, SH ;

Wei, J ;

Lin, JY .

CHEMICAL PHYSICS LETTERS, 2004, 400 (4-6) :578-582

[23] Amperometric glucose biosensor based on self-assembling glucose oxidase on carbon nanotubes [J].

Liu, GD ;

Lin, YH .

ELECTROCHEMISTRY COMMUNICATIONS, 2006, 8 (02) :251-256

[24] The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix [J].

Liu, Y ;

Wang, MK ;

Zhao, F ;

Xu, ZA ;

Dong, SJ .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (06) :984-988

[25]

LOPEZAVILA V, 1997, ANAL CHEM, V69, P289

[26] Ferrocenyl silicon-based dendrimers as mediators in amperometric biosensors [J].

Losada, J ;

Cuadrado, I ;

Moran, M ;

Casado, CM ;

Alonso, B ;

Barranco, M .

ANALYTICA CHIMICA ACTA, 1997, 338 (03) :191-198

[27] Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode [J].

Luo, HX ;

Shi, ZJ ;

Li, NQ ;

Gu, ZN ;

Zhuang, QK .

ANALYTICAL CHEMISTRY, 2001, 73 (05) :915-920

[28] Ferrocene-conjugated m-phenylenediamine conducting polymer-incorporated peroxidase biosensors [J].

Mulchandani, A ;

Pan, ST .

ANALYTICAL BIOCHEMISTRY, 1999, 267 (01) :141-147

[29] Fast electron transfer kinetics on multiwalled carbon nanotube microbundle electrodes [J].

Nugent, JM ;

Santhanam, KSV ;

Rubio, A ;

Ajayan, PM .

NANO LETTERS, 2001, 1 (02) :87-91

[30] Tethered mediator biosensor. Mediated electron transfer between redox enzyme and electrode via ferrocene anchored to electrode surface with long poly(oxyethylene) chain [J].

Okawa, Y ;

Nagano, M ;

Hirota, S ;

Kobayashi, H ;

Ohno, T ;

Watanabe, M .

BIOSENSORS & BIOELECTRONICS, 1999, 14 (02) :229-235

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