Purification of wheat flour high-Mr glutenin subunits by Reactive Red 120-Agarose and Reactive Yellow 86-Agarose resins

被引:15
作者
Fisichella, S
Alberghina, G
Amato, ME
Lafiandra, D
Mantarro, D
Palermo, A
Savarino, A
Scarlata, G
机构
[1] Univ Catania, Dept Chem, I-95125 Catania, Italy
[2] Univ Tuscia, Dept Agrobiol & Agrochem, I-01100 Viterbo, Italy
关键词
wheat; high-M-r; glutenin subunits; dye-ligand chromatography; Reactive Red 120; Reactive Yellow 86;
D O I
10.1016/S0733-5210(03)00005-5
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Two reactive textile dyes were employed to purify some high-M-r glutenin subunit mixtures by dye-ligand chromatography. In particular, the use of the triazine dye Reactive Red 120 coupled to Agarose bed provided an efficient method to purify high-M-r glutenin subunit mixtures, showing high binding capacity and high selectivity for these proteins and assuring a recovery of about 80%. On the other hand, the Reactive Yellow 86 had weaker interaction and a lower selectivity than Red 120 with a recovery of about 60%. However, this dye allowed the separation of some subunits that were not separated by reactive Red 120. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:77 / 85
页数:9
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