Purification of wheat flour high-Mr glutenin subunits by Reactive Red 120-Agarose and Reactive Yellow 86-Agarose resins

Two reactive textile dyes were employed to purify some high-M-r glutenin subunit mixtures by dye-ligand chromatography. In particular, the use of the triazine dye Reactive Red 120 coupled to Agarose bed provided an efficient method to purify high-M-r glutenin subunit mixtures, showing high binding capacity and high selectivity for these proteins and assuring a recovery of about 80%. On the other hand, the Reactive Yellow 86 had weaker interaction and a lower selectivity than Red 120 with a recovery of about 60%. However, this dye allowed the separation of some subunits that were not separated by reactive Red 120. (C) 2003 Elsevier Science Ltd. All rights reserved.