Single-molecule analysis of RNA polymerase transcription

被引：98

作者：

Bai, Lu ^{[1
]}

Santangelo, Thomas J.

Wang, Michelle D.

机构：

[1] Cornell Univ, Atom & Solid State Phys Lab, Dept Phys, Ithaca, NY 14853 USA

[2] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2006年 / 35卷

关键词：

initiation; elongation; kinetics; mechanism; pausing;

D O I：

10.1146/annurev.biophys.35.010406.150153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.

引用

页码：343 / 360

页数：18

共 77 条

[1] Picocalorimetry of transcription by RNA polymerase
Abbondanzieri, EA
Shaevitz, JW
Block, SM
[J]. BIOPHYSICAL JOURNAL, 2005, 89 (06) : L61 - L63
[2] Molecular mechanism of transcription inhibition by peptide antibiotic microcin J25
Adelman, K
Yuzenkova, J
La Porta, A
Zenkin, N
Lee, J
Lis, JT
Borukhov, S
Wang, MD
Severinov, K
[J]. MOLECULAR CELL, 2004, 14 (06) : 753 - 762
[3] Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior
Adelman, K
La Porta, A
Santangelo, TJ
Lis, JT
Roberts, JW
Wang, MD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) : 13538 - 13543
[4] Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
Artsimovitch, I
Landick, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) : 7090 - 7095
[5] Sequence-dependent kinetic model for transcription elongation by RNA polymerase
Bai, L
Shundrovsky, A
Wang, MD
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2004, 344 (02) : 335 - 349
[6] DIFFUSION-DRIVEN MECHANISMS OF PROTEIN TRANSLOCATION ON NUCLEIC-ACIDS .1. MODELS AND THEORY
BERG, OG
WINTER, RB
VONHIPPEL, PH
[J]. BIOCHEMISTRY, 1981, 20 (24) : 6929 - 6948
[7] TRANSCRIPT CLEAVAGE FACTORS FROM ESCHERICHIA-COLI
BORUKHOV, S
SAGITOV, V
GOLDFARB, A
[J]. CELL, 1993, 72 (03) : 459 - 466
[8] Facilitated target location on DNA by individual Escherichia coli RNA polymerase molecules observed with the scanning force microscope operating in liquid
Bustamante, C
Guthold, M
Zhu, XS
Yang, GL
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) : 16665 - 16668
[9] Insights into transcription: structure and function of single-subunit DNA-dependent RNA polymerases
Cheetham, GMT
Steitz, TA
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (01) : 117 - 123
[10] Multisubunit RNA polymerases
Cramer, P
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (01) : 89 - 97

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