Selection and fine mapping of chromosome-specific cDNAs: Application to human chromosome 1

We have developed a methodology for identification and fine mapping of chromosome-specific transcripts, Combining digestion of DNA with different restriction enzymes, ligation to ''bubble'' linkers, and PCR amplification from Alu and ''bubble'' primers, we have synthesized human chromosome 1-specific sequences from DNA of a somatic cell hybrid, A9Neol, After hybridization to human fetal brain cDNA, we could efficiently capture chromosome 1-specific cDNAs, The cDNAs were sequenced and used as probes in hybridizations to high-density filters containing the arrayed CEPH Mega-YAC library and to the arrayed cDNA library from infant brain made by B. Soares, which has been extensively sequenced. By this approach we have been able to select chromosome 1-specific cDNAs, to map them to chromosome 1 YAC contigs, and to identify and map corresponding longer cDNAs and ESTs. (C) 1996 Academic Press, Inc.