The effect of bioactive hydrogels on the secretion of extracellular matrix molecules by valvular interstitial cells

被引：52

作者：

Shah, Darshita N. ^{[1
]}

Recktenwall-Work, Sarah M. ^{[1
]}

Anseth, Kristi S. ^{[1
,2
]}

机构：

[1] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA

[2] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA

来源：

BIOMATERIALS | 2008年 / 29卷 / 13期

关键词：

hydrogel; degradation; extracellular matrix; hyaluronic acid; heart valve;

D O I：

10.1016/j.biomaterials.2008.01.001

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Valvular interstitial cells (VICs) were encapsulated in enzymatically degradable, crosslinked hydrogels formed from hyaluronic acid (HA) and poly(ethylene glycol) (PEG) macromolecular monomers. Titration of PEG with HA allowed for the synthesis of gels with a broad compositional spectrum, leading to a range of degradation behavior upon exposure to bovine testes hyaluronidase. The rate of mass loss and release of HA fragments from the copolymer gels depended on the PEG content of the network. These hydrogels were shown to have the dual function of permitting the diffusion of ECM elaborated by 3D cultured VICs and promoting the development of a specific matrix composition. Initial cleavage of hydrogel crosslinks, prior to network mass loss, permit the diffusion of collagen, while later stages of degradation promote elastin elaboration and suppress collagen production due to HA fragment release. Exogenous HA delivery through the cell culture media further demonstrated the utility of delivered HA on manipulating the secretory properties of encapsulated VICs. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2060 / 2072

页数：13

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