Stiffness-mediated adhesion of cervical cancer cells to soft hydrogel films

被引：26

作者：

Best, James P. ^{[1
]}

Javed, Sameen ^{[1
]}

Richardson, Joseph J. ^{[1
]}

Cho, Kwun Lun ^{[1
]}

Kamphuis, Marloes M. J. ^{[1
]}

Caruso, Frank ^{[1
]}

机构：

[1] Univ Melbourne, Dept Chem & Biomol Engn, Parkville, Vic 3010, Australia

来源：

SOFT MATTER | 2013年 / 9卷 / 18期

基金：

澳大利亚研究理事会;

关键词：

POLYELECTROLYTE MULTILAYER FILMS; SUBSTRATE STIFFNESS; MATRIX STIFFNESS; TUMOR-CELLS; GROWTH; TISSUE; ELASTICITY; MODULUS; GELS;

D O I：

10.1039/c3sm50587a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

We report engineered hydrogel thin-films with varying degrees of covalent crosslinking, which demonstrate enhanced HeLa cell adhesion with decreasing film stiffness. This trend is contrary to previous findings for tumour cell adhesion on hydrogel substrates, and is attributed to the extremely soft nature of the films studied, allowing for a greater cell-film contact area and the development of adhesive focal contacts. Adhesion based on mechanical tuning of the film was decoupled from chemical effects through characterisation and analysis of film surface roughness, hydrophobicity and charge.

引用

页码：4580 / 4584

页数：5

共 33 条

[1]

[Anonymous], CONTAMINATION CLEANI

[2]

Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures [J].