Age-Dependent Impaired Neurogenic Differentiation Capacity of Dental Stem Cell is Associated with Wnt/β-Catenin Signaling

被引:82
作者
Feng, Xingmei [1 ]
Xing, Jing [1 ]
Feng, Guijuan [1 ]
Sang, Aimin [2 ]
Shen, Biyu [3 ]
Xu, Yue [2 ]
Jiang, Jinxia [3 ]
Liu, Suzhe [4 ]
Tan, Wei [3 ]
Gu, Zhifeng [3 ]
Li, Liren [4 ]
机构
[1] Nantong Univ, Affiliated Hosp, Dept Stomatol, Nantong 226001, Jiangsu, Peoples R China
[2] Nantong Univ, Affiliated Hosp, Dept Ophthalmol, Nantong 226001, Jiangsu, Peoples R China
[3] Nantong Univ, Affiliated Hosp, Dept Rheumatol, Nantong 226001, Jiangsu, Peoples R China
[4] Nantong Univ, Affiliated Hosp, Dept Div Gastroenterol & Hepatol, Nantong 226001, Jiangsu, Peoples R China
关键词
Age-dependent; Dental pulp stem cells; Neurogenic differentiation; Wnt/beta-Catenin signaling; BONE-MARROW; IN-VITRO; NEURAL DIFFERENTIATION; PULP; SENESCENCE; HEART;
D O I
10.1007/s10571-013-9965-0
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Two kinds of dental stem cells (DSCs), dental pulp stem cells (DPSCs) and stem cells from human-exfoliated deciduous teeth (SHED), have been identified as novel populations of mesenchymal stem cells that can be induced to differentiate into osteoblasts, chondrocytes, adipocytes, and neuron-like cells in vitro. As we know, both of them originate from the neural crest, but have distinct characteristics and functions in vitro and in vivo. The regeneration potential of DSCs declines with advanced age; however, the mechanism of the impaired potential in DSCs has not been fully explored. In this study, we investigated whether declined neurogenic differentiation capacity is associated with an altered expression of Wnt signaling-related proteins in vitro. We compared stem cells isolated from human dental pulp in two age groups: the exfoliated deciduous teeth (5-12 years), and the third permanent teeth (45-50 years). We found that the expression levels of neuron markers, such as beta III-tubulin, microtubule-associated protein 2(MAP2), tyrosine hydroxylase (TH), and Nestin were lower in the DPSCs group compared with that in the SHED group; however, in supplementation with human recombinant Wnt1 in the medium, the DPSCs were prone to neural differentiation and expressed higher levels of neurogenic markers. In summary, our study demonstrated that Wnt/beta-catenin signaling may play a vital role in the age-dependent neural differentiation of DSCs. Therefore, DSCs may provide an ideal source of stem cells that can further extend their therapeutic application in nerve injury and neurodegenerative diseases.
引用
收藏
页码:1023 / 1031
页数:9
相关论文
共 41 条
[1]
Implanted Adult Human Dental Pulp Stem Cells Induce Endogenous Axon Guidance [J].
Arthur, Agnieszka ;
Shi, Songtao ;
Zannettino, Andrew C. W. ;
Fujii, Nobutaka ;
Gronthos, Stan ;
Koblar, Simon A. .
STEM CELLS, 2009, 27 (09) :2229-2237
[2]
Proliferation, differentiation and self-renewal of osteoprogenitors in vertebral cell populations from aged and young female rats [J].
Bellows, CG ;
Pei, W ;
Jia, Y ;
Heersche, JNM .
MECHANISMS OF AGEING AND DEVELOPMENT, 2003, 124 (06) :747-757
[3]
The signals and pathways activating cellular senescence [J].
Ben-Porath, I ;
Weinberg, RA .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2005, 37 (05) :961-976
[4]
When cells get stressed: an integrative view of cellular senescence [J].
Ben-Porath, I ;
Weinberg, RA .
JOURNAL OF CLINICAL INVESTIGATION, 2004, 113 (01) :8-13
[5]
Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis [J].
Brack, Andrew S. ;
Conboy, Michael J. ;
Roy, Sudeep ;
Lee, Mark ;
Kuo, Calvin J. ;
Keller, Charles ;
Rando, Thomas A. .
SCIENCE, 2007, 317 (5839) :807-810
[6]
Senescent cells, tumor suppression, and organismal aging: Good citizens, bad neighbors [J].
Campisi, J .
CELL, 2005, 120 (04) :513-522
[7]
Stem Cell Aging and Aberrant Differentiation within the Niche [J].
Choi, Jinkuk ;
Artandi, Steven .
CELL STEM CELL, 2009, 5 (01) :6-8
[8]
Wnt/β-catenin signaling in development and disease [J].
Clevers, Hans .
CELL, 2006, 127 (03) :469-480
[9]
Which way does the Wnt blow? Exploring the duality of canonical Wnt signaling on cellular aging [J].
DeCarolis, Nathan A. ;
Wharton, Keith A., Jr. ;
Eisch, Amelia J. .
BIOESSAYS, 2008, 30 (02) :102-106
[10]
Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].
Gronthos, S ;
Mankani, M ;
Brahim, J ;
Robey, PG ;
Shi, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630