T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains

被引:52
作者
Brdicka, T
Cerny, J
Horejsí, V
机构
[1] Acad Sci Czech Republ, Inst Genet Mol, Prague 14220 4, Czech Republic
[2] Charles Univ, Fac Sci, Prague, Czech Republic
关键词
D O I
10.1006/bbrc.1998.8857
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stimulation of human T cell line Jurkat results in rapid tyrosine phosphorylation of a 35-38 kDa protein that is found in large and buoyant detergent-resistant membrane microdomains containing also glycosylphosphatidylinositol (GPI)-anchored proteins, glycolipids and Src-family protein tyrosine kinases ("GPI-microdomains"). The pp35-38 was found to be identical to LAT, a recently cloned key component of the T-cell receptor signalling pathway. Moreover, a modified form of protein tyrosine kinase Lck (pp60) was newly detected in the GPI-microdomains of the anti-CD3-stimulated Jurkat cells. These data support the idea that GPI-microdomains play important roles in immunoreceptor signalling. (C) 1998 Academic Press.
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页码:356 / 360
页数:5
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