In vitro selection of phosphorothiolated aptamers

被引：39

作者：

Jhaveri, S

Olwin, B

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Inst Mol & Cellular Biol, Austin, TX 78712 USA

[2] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1998年 / 8卷 / 17期

关键词：

D O I：

10.1016/S0960-894X(98)00414-4

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A pool of RNA molecules that contained exclusively phosphorothioate internucleoside linkages was used as a starting point for the selection of aptamers that bind to basic fibroblast growth factor (bFGF), and appear to act as heparin mimics. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：2285 / 2290

页数：6

相关论文

共 11 条

[1]

Conrad RC, 1996, METHOD ENZYMOL, V267, P336

[2] Post-SELEX combinatorial optimization of aptamers [J].

Eaton, BE ;

Gold, L ;

Hicke, BJ ;

Janjic, N ;

Jucker, FM ;

Sebesta, DP ;

Tarasow, TM ;

Willis, MC ;

Zichi, DA .

BIOORGANIC & MEDICINAL CHEMISTRY, 1997, 5 (06) :1087-1096

[3] INHIBITION OF HIGH-AFFINITY BASIC FIBROBLAST GROWTH-FACTOR BINDING BY OLIGONUCLEOTIDES [J].

FENNEWALD, SM ;

RANDO, RF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21718-21721

[4] NUCLEASE-RESISTANT NUCLEIC-ACID LIGANDS TO VASCULAR-PERMEABILITY FACTOR VASCULAR ENDOTHELIAL GROWTH-FACTOR [J].

GREEN, LS ;

JELLINEK, D ;

BELL, C ;

BEEBE, LA ;

FEISTNER, BD ;

GILL, SC ;

JUCKER, FM ;

JANJIC, N .

CHEMISTRY & BIOLOGY, 1995, 2 (10) :683-695

[5] HIGH-AFFINITY RNA LIGANDS TO BASIC FIBROBLAST GROWTH-FACTOR INHIBIT RECEPTOR-BINDING [J].

JELLINEK, D ;

LYNOTT, CK ;

RIFKIN, DB ;

JANJIC, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :11227-11231

[6] POTENT 2'-AMINO-2'-DEOXYPYRIMIDINE RNA INHIBITORS OF BASIC FIBROBLAST GROWTH-FACTOR [J].

JELLINEK, D ;

GREEN, LS ;

BELL, C ;

LYNOTT, CK ;

GILL, N ;

VARGEESE, C ;

KIRSCHENHEUTER, G ;

MCGEE, DPC ;

ABESINGHE, P ;

PIEKEN, WA ;

SHAPIRO, R ;

RIFKIN, DB ;

MOSCATELLI, D ;

JANJIC, N .

BIOCHEMISTRY, 1995, 34 (36) :11363-11372

[7] Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor [J].

Pagratis, NC ;

Bell, C ;

Chang, YF ;

Jennings, S ;

Fitzwater, T ;

Jellinek, D ;

Dang, C .

NATURE BIOTECHNOLOGY, 1997, 15 (01) :68-73

[8]

RAPRAEGER AC, 1994, METHOD ENZYMOL, V245, P219

[9] Exploiting the potential of antisense: Beyond phosphorothioate oligodeoxynucleotides [J].

Stein, CA .

CHEMISTRY & BIOLOGY, 1996, 3 (05) :319-323

[10] PHOSPHOROTHIOATE-CONTAINING RNAS SHOW MESSENGER-RNA ACTIVITY IN THE PROKARYOTIC TRANSLATION SYSTEMS INVITRO [J].

UEDA, T ;

TOHDA, H ;

CHIKAZUMI, N ;

ECKSTEIN, F ;

WATANABE, K .

NUCLEIC ACIDS RESEARCH, 1991, 19 (03) :547-552