Behind the scenes of a small RNA gene-silencing pathway

被引：17

作者：

Ku, Gregory ^{[1
,2
]}

McManus, Michael T. ^{[1
,3
]}

机构：

[1] Univ Calif San Francisco, UCSF Diabet Ctr, San Francisco, CA 94122 USA

[2] Univ Calif San Francisco, Dept Med, Div Endocrinol & Metab, San Francisco, CA 94122 USA

[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94122 USA

来源：

HUMAN GENE THERAPY | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1089/hum.2007.1226

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference (RNAi) is a sequence-specific gene-silencing phenomenon mediated by double-stranded RNA. RNAi is made possible through the activity of an evolutionarily conserved RNA-protein machinery that is utilized by microRNAs (miRNAs), endogenously produced 21- to 23-nucleotide noncoding RNAs. As RNA-based therapeutics come of age, understanding the miRNA pathway and the mechanism of posttranscriptional gene silencing is paramount. Studies have pointed to a critical role for miRNAs in human development and disease and revealed an unanticipated complexity in the mechanisms of regulation of gene expression. This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA- based treatments.

引用

收藏

页码：17 / 26

页数：10

相关论文

共 119 条

[101] Regulation of the germinal center response by microRNA-155 [J].

Thai, To-Ha ;

Calado, Dinis Pedro ;

Casola, Stefano ;

Ansel, K. Mark ;

Xiao, Changchun ;

Xue, Yingzi ;

Murphy, Andrew ;

Frendewey, David ;

Valenzuela, David ;

Kutok, Jeffery L. ;

Schmidt-Supprian, Marc ;

Rajewsky, Nikolaus ;

Yancopoulos, George ;

Rao, Anjana ;

Rajewsky, Klaus .

SCIENCE, 2007, 316 (5824) :604-608

[102] Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation [J].

Thermann, Rolf ;

Hentze, Matthias W. .

NATURE, 2007, 447 (7146) :875-878

[103] A protein sensor for siRNA asymmetry [J].

Tomari, Y ;

Matranga, C ;

Haley, B ;

Martinez, N ;

Zamore, PD .

SCIENCE, 2004, 306 (5700) :1377-1380

[104] Targeted mRNA degradation by double-stranded RNA in vitro [J].

Tuschl, T ;

Zamore, PD ;

Lehmann, R ;

Bartel, DP ;

Sharp, PA .

GENES & DEVELOPMENT, 1999, 13 (24) :3191-3197

[105] FLAVONOID GENES IN PETUNIA - ADDITION OF A LIMITED NUMBER OF GENE COPIES MAY LEAD TO A SUPPRESSION OF GENE-EXPRESSION [J].

VANDERKROL, AR ;

MUR, LA ;

BELD, M ;

MOL, JNM ;

STUITJE, AR .

PLANT CELL, 1990, 2 (04) :291-299

[106] Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system [J].

Wakiyama, Motoaki ;

Takimoto, Koji ;

Ohara, Osamu ;

Yokoyama, Shigeyuki .

GENES & DEVELOPMENT, 2007, 21 (15) :1857-1862

[107] Recapitulation of short RNA-directed translational gene silencing in vitro [J].

Wang, Bingbing ;

Love, Tara M. ;

Call, Matthew E. ;

Doench, John G. ;

Novina, Carl D. .

MOLECULAR CELL, 2006, 22 (04) :553-560

[108] DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal [J].

Wang, Yangming ;

Medvid, Rostislav ;

Melton, Collin ;

Jaenisch, Rudolf ;

Blelloch, Robert .

NATURE GENETICS, 2007, 39 (03) :380-385

[109] POSTTRANSCRIPTIONAL REGULATION OF THE HETEROCHRONIC GENE LIN-14 BY LIN-4 MEDIATES TEMPORAL PATTERN-FORMATION IN C-ELEGANS [J].

WIGHTMAN, B ;

HA, I ;

RUVKUN, G .

CELL, 1993, 75 (05) :855-862

[110] MicroRNAs direct rapid deadenylation of mRNA [J].

Wu, LG ;

Fan, JH ;

Belasco, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (11) :4034-4039

← 3 4 5 6 7 8 9 10 11 12 →