Identification of Noncoding Transcripts from within CENP-A Chromatin at Fission Yeast Centromeres

被引：97

作者：

Choi, Eun Shik ^{[2
,3
]}

Stralfors, Annelie ^{[1
]}

Castillo, Araceli G. ^{[2
,3
]}

Durand-Dubief, Mickael ^{[1
]}

Ekwall, Karl ^{[1
]}

Allshire, Robin C. ^{[2
,3
]}

机构：

[1] Karolinska Inst, Novum, Dept Biosci & Nutr, Ctr Biosci, S-14157 Huddinge, Sweden

[2] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[3] Univ Edinburgh, Inst Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 26期

基金：

英国惠康基金; 瑞典研究理事会;

关键词：

COMPREHENSIVE ANALYSIS; EPIGENETIC REGULATION; RNA; HETEROCHROMATIN; PROTEIN; GENE; KINETOCHORES; COMPONENTS; TURNOVER; DYNAMICS;

D O I：

10.1074/jbc.M111.228510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The histone H3 variant CENP-A is the most favored candidate for an epigenetic mark that specifies the centromere. In fission yeast, adjacent heterochromatin can direct CENP-A(Cnp1) chromatin establishment, but the underlying features governing where CENP-A(Cnp1) chromatin assembles are unknown. We show that, in addition to centromeric regions, a low level of CENP-A(Cnp1) associates with gene promoters where histone H3 is depleted by the activity of the Hrp1(Chd1) chromatin-remodeling factor. Moreover, we demonstrate that noncoding RNAs are transcribed by RNA polymerase II (RNAPII) from CENP-A(Cnp1) chromatin at centromeres. These analyses reveal a similarity between centromeres and a subset of RNAPII genes and suggest a role for remodeling at RNAPII promoters within centromeres that influences the replacement of histone H3 with CENP-A(Cnp1).

引用

页码：23600 / 23607

页数：8

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