Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection

被引：95

作者：

González-Peñas, E

Leache, C

Viscarret, M

de Obanos, AP

Araguás, C

de Cerain, AL

机构：

[1] Univ Navarra, Organ & Pharmaceut Chem Dept, Fac Pharm, Pamplona 31008, Spain

[2] Univ Navarra, Food Sci & Toxicol Dept, Fac Pharm, Pamplona 31008, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1025卷 / 02期

关键词：

wine; liquid-phase microextraction; mycotoxins; ochratoxin A;

D O I：

10.1016/j.chroma.2003.10.113

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid-liquid microextraction technique (LPME) has been applied to the extraction of ochratoxin A (OTA) from wine prior to its quantification by HPLC-fluorescence detection. OTA was extracted from wine, through l-octanol immobilized in the pores of a porous hollow fiber, and introduced into l-octanol inside the fiber. Recovery was 77%. The method was adequate for quantification of OTA in wine at levels within the range 0.25-10 ng/ml with a LOD of 0.2 ng/ml, and can be a simple and inexpensive alternative to the use of innumoaffinity columns in order to quantify OTA levels in wine. (C) 2003 Elsevier B.V. All rights reserved.

引用

收藏

页码：163 / 168

页数：6

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