Clonal transmission of ESBL-producing Klebsiella spp. at a university hospital in Brazil

被引：20

作者：

Minarini, Luciene A. R. ^{[1
]}

Climaco, Eduardo C. ^{[1
]}

Guimaraes, Daniela B. ^{[1
]}

Ferreira, Joseane C. ^{[1
]}

Palazzo, Izabel C. V. ^{[1
]}

Martinez, Roberto ^{[2
]}

Darini, Ana Lucia C. ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-14040903 Ribeirao Preto, SP, Brazil

来源：

CURRENT MICROBIOLOGY | 2008年 / 56卷 / 06期

关键词：

D O I：

10.1007/s00284-008-9129-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The present study was designed to determine the prevalence and extended-spectrum beta-lactamase (ESBL) types in clinical isolates of Klebsiella spp. at a university hospital located in the Brazilian southern region (Ribeirao Preto, Sao Paulo) as well as their antibiotic susceptibility and genetic profiles. This study included 147 non-repeat Klebsiella spp. isolates collected from January to June 2000, of which 23 K. pneumoniae and 8 K. oxytoca were selected as ESBL producers by using the Oxoid combination disk method and Etest ESBL strip. beta-lactamases were characterized by IEF, PCR and sequencing assays using primers for ESBL genes. Antibiotic susceptibility was evaluated by MicroScan system. Dissemination of two major clones of ESBL-producing Klebsiella spp. occurred in the hospital. According to the results obtained in this study there was a clonal spread of CTX-M-producing K. oxytoca in five clinics and dissemination of ESBL-producing K. pneumoniae in the nursery and pediatrics wards.

引用

页码：587 / 591

页数：5

共 20 条

[1] A novel CTX-M β-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil [J].