RNA interference in functional genomics and medicine

被引：41

作者：

Kim, VN

机构：

[1] Seoul Natl Univ, Inst Mol Biol & Genet, Gwanak Gu, Seoul 151742, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea

来源：

JOURNAL OF KOREAN MEDICAL SCIENCE | 2003年 / 18卷 / 03期

关键词：

RNA interference (RNAi); RNA; small interfering (siRNA); microRNAs (miRNA); small hairpin RNA (shRNA); mRNA degradation; translation; functional genomics; gene therapy;

D O I：

10.3346/jkms.2003.18.3.309

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA (dsRNA). Being a highly specific and efficient knockdown technique, RNAi not only provides a powerful tool for functional genomics but also holds a promise for gene therapy. The key player in RNAi is small RNA (similar to22-nt) termed siRNA. Small RNAs are involved not only in RNAi but also in basic cellular processes, such as developmental control and heterochromatin formation. The interesting biology as well as the remarkable technical value has been drawing widespread attention to this exciting new field.

引用

收藏

页码：309 / 318

页数：10

相关论文

共 114 条

[1] Lentiviral-mediated RNA interference [J].

Abbas-Terki, T ;

Blanco-Bose, W ;

Déglon, N ;

Pralong, W ;

Aebischer, P .

HUMAN GENE THERAPY, 2002, 13 (18) :2197-2201

[2] RNA interference may be more potent than antisense RNA in human cancer cell lines [J].

Aoki, Y ;

Cioca, DP ;

Oidaira, H ;

Kamiya, J ;

Kiyosawa, K .

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2003, 30 (1-2) :96-102

[3] Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes [J].

Ashrafi, K ;

Chang, FY ;

Watts, JL ;

Fraser, AG ;

Kamath, RS ;

Ahringer, J ;

Ruvkun, G .

NATURE, 2003, 421 (6920) :268-272

[4] Retroviral delivery of small interfering RNA into primary cells [J].

Barton, GM ;

Medzhitov, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14943-14945

[5] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[6] Regulating axon branch stability: The role of p190 RhoGAP in repressing a retraction signaling pathway [J].

Billuart, P ;

Winter, CG ;

Maresh, A ;

Zhao, XS ;

Luo, LQ .

CELL, 2001, 107 (02) :195-207

[7] Blocking oncogenes in malignant cells by RNA interference - New hope for a highly specific cancer treatment? [J].

Borkhardt, A .

CANCER CELL, 2002, 2 (03) :167-168

[8] bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila [J].

Brennecke, J ;

Hipfner, DR ;

Stark, A ;

Russell, RB ;

Cohen, SM .

CELL, 2003, 113 (01) :25-36

[9] Stable suppression of tumorigenicity by virus-mediated RNA interference [J].

Brummelkamp, TR ;

Bernards, R ;

Agami, R .

CANCER CELL, 2002, 2 (03) :243-247

[10] A system for stable expression of short interfering RNAs in mammalian cells [J].

Brummelkamp, TR ;

Bernards, R ;

Agami, R .

SCIENCE, 2002, 296 (5567) :550-553

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