RNA interference in functional genomics and medicine

被引：41

作者：

Kim, VN

机构：

[1] Seoul Natl Univ, Inst Mol Biol & Genet, Gwanak Gu, Seoul 151742, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea

来源：

JOURNAL OF KOREAN MEDICAL SCIENCE | 2003年 / 18卷 / 03期

关键词：

RNA interference (RNAi); RNA; small interfering (siRNA); microRNAs (miRNA); small hairpin RNA (shRNA); mRNA degradation; translation; functional genomics; gene therapy;

D O I：

10.3346/jkms.2003.18.3.309

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA (dsRNA). Being a highly specific and efficient knockdown technique, RNAi not only provides a powerful tool for functional genomics but also holds a promise for gene therapy. The key player in RNAi is small RNA (similar to22-nt) termed siRNA. Small RNAs are involved not only in RNAi but also in basic cellular processes, such as developmental control and heterochromatin formation. The interesting biology as well as the remarkable technical value has been drawing widespread attention to this exciting new field.

引用

收藏

页码：309 / 318

页数：10

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