RNA interference in functional genomics and medicine

被引：41

作者：

Kim, VN

机构：

[1] Seoul Natl Univ, Inst Mol Biol & Genet, Gwanak Gu, Seoul 151742, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea

来源：

JOURNAL OF KOREAN MEDICAL SCIENCE | 2003年 / 18卷 / 03期

关键词：

RNA interference (RNAi); RNA; small interfering (siRNA); microRNAs (miRNA); small hairpin RNA (shRNA); mRNA degradation; translation; functional genomics; gene therapy;

D O I：

10.3346/jkms.2003.18.3.309

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA (dsRNA). Being a highly specific and efficient knockdown technique, RNAi not only provides a powerful tool for functional genomics but also holds a promise for gene therapy. The key player in RNAi is small RNA (similar to22-nt) termed siRNA. Small RNAs are involved not only in RNAi but also in basic cellular processes, such as developmental control and heterochromatin formation. The interesting biology as well as the remarkable technical value has been drawing widespread attention to this exciting new field.

引用

收藏

页码：309 / 318

页数：10

相关论文

共 114 条

[41] Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C-elegans [J].

Ketting, RF ;

Fischer, SEJ ;

Bernstein, E ;

Sijen, T ;

Hannon, GJ ;

Plasterk, RHA .

GENES & DEVELOPMENT, 2001, 15 (20) :2654-2659

[42] Femcoat, a novel eggshell protein in Drosophila:: functional analysis by double stranded RNA interference [J].

Kim, C ;

Han, K ;

Kim, J ;

Yi, JS ;

Kim, C ;

Yim, J ;

Kim, YJ ;

Kim-Ha, J .

MECHANISMS OF DEVELOPMENT, 2002, 110 (1-2) :61-70

[43] A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans [J].

Knight, SW ;

Bass, BL .

SCIENCE, 2001, 293 (5538) :2269-2271

[44] Antisense RNA: Function and fate of duplex RNA in cells of higher eukaryotes [J].

Kumar, M ;

Carmichael, GG .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) :1415-+

[45] New microRNAs from mouse and human [J].

Lagos-Quintana, M ;

Rauhut, R ;

Meyer, J ;

Borkhardt, A ;

Tuschl, T .

RNA, 2003, 9 (02) :175-179

[46] Identification of tissue-specific microRNAs from mouse [J].

Lagos-Quintana, M ;

Rauhut, R ;

Yalcin, A ;

Meyer, J ;

Lendeckel, W ;

Tuschl, T .

CURRENT BIOLOGY, 2002, 12 (09) :735-739

[47] Identification of novel genes coding for small expressed RNAs [J].

Lagos-Quintana, M ;

Rauhut, R ;

Lendeckel, W ;

Tuschl, T .

SCIENCE, 2001, 294 (5543) :853-858

[48] Micro RNAs are complementary to 3′ UTR sequence motifs that mediate negative post-transcriptional regulation [J].

Lai, EC .

NATURE GENETICS, 2002, 30 (04) :363-364

[49] An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans [J].

Lau, NC ;

Lim, LP ;

Weinstein, EG ;

Bartel, DP .

SCIENCE, 2001, 294 (5543) :858-862

[50] Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells [J].

Lee, NS ;

Dohjima, T ;

Bauer, G ;

Li, HT ;

Li, MJ ;

Ehsani, A ;

Salvaterra, P ;

Rossi, J .

NATURE BIOTECHNOLOGY, 2002, 20 (05) :500-505

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