RNA Polymerase II cluster dynamics predict mRNA output in living cells

被引：202

作者：

Cho, Won-Ki ^{[1
]}

Jayanth, Namrata ^{[1
]}

English, Brian P. ^{[2
]}

Inoue, Takuma ^{[1
]}

Andrews, J. Owen ^{[1
]}

Conway, William ^{[1
]}

Grimm, Jonathan B. ^{[2
]}

Spille, Jan-Hendrik ^{[1
]}

Lavis, Luke D. ^{[2
]}

Lionnet, Timothee ^{[2
]}

Cisse, Ibrahim I. ^{[1
]}

机构：

[1] MIT, Dept Phys, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[2] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, MD USA

来源：

ELIFE | 2016年 / 5卷

基金：

美国国家卫生研究院;

关键词：

REAL-TIME OBSERVATION; TRANSCRIPTION FACTORIES; NUCLEAR ARCHITECTURE; FLUORESCENT PROTEIN; GENE-EXPRESSION; ORGANIZATION; SITES; LOCALIZATION; ELONGATION; GENOME;

D O I：

10.7554/eLife.13617

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II(Pol II) clusters at a gene locus. For endogenous beta-actin genes in mouse embryonic fibroblasts, we observe that short-lived (similar to 8 s) Pol II clusters correlate with basal mRNA output. During serum stimulation, a stereotyped increase in Pol II cluster lifetime correlates with a proportionate increase in the number of mRNAs synthesized. Our findings suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.

引用

页数：31

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