A forward-genetic approach for analysis of the immune system

被引：15

作者：

Appleby, MW ^{[1
]}

Ramsdell, F ^{[1
]}

机构：

[1] Celltech R & D Inc, Discovery Biol, Bothell, WA 98021 USA

来源：

NATURE REVIEWS IMMUNOLOGY | 2003年 / 3卷 / 06期

关键词：

D O I：

10.1038/nri1109

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The completion of the genome sequences of both humans and mice challenges biologists to determine gene function on a vast, whole-organism scale. Both phenotype-based ('forward') and gene-based ('reverse') strategies are being developed to approach this issue. Forward-genetic approaches, however, provide the unique ability of assigning function to genes in an unbiased, global manner that is independent of previous assumptions about gene function. In this article, we compare various genetic technologies for their potential role in dissecting immune-system development and function, with particular emphasis on the worldwide efforts that use chemical mutagenesis as a forward-genetic strategy.

引用

页码：463 / 471

页数：9

共 28 条

[1] ENU mutagenesis: a work in progress [J].

Beier, DR .

PHYSIOLOGICAL GENOMICS, 2002, 11 (03) :111-113

[2] Combining mutagenesis and genomics in the mouse - Closing the phenotype gap [J].

Brown, SDM ;

Peters, J .

TRENDS IN GENETICS, 1996, 12 (11) :433-435

[3] Genome-wide, large-scale production of mutant mice by ENU mutagenesis [J].

de Angelis, MH ;

Flaswinkel, H ;

Fuchs, H ;

Rathkolb, B ;

Soewarto, D ;

Marschall, S ;

Heffner, S ;

Pargent, W ;

Wuensch, K ;

Jung, M ;

Reis, A ;

Richter, T ;

Alessandrini, F ;

Jakob, T ;

Fuchs, E ;

Kolb, H ;

Kremmer, E ;

Schaeble, K ;

Rollinski, B ;

Roscher, A ;

Peters, C ;

Meitinger, T ;

Strom, T ;

Steckler, T ;

Holsboer, F ;

Klopstock, T ;

Gekeler, F ;

Schindewolf, C ;

Jung, T ;

Avraham, K ;

Behrendt, H ;

Ring, J ;

Zimmer, A ;

Schughart, K ;

Pfeffer, K ;

Wolf, E ;

Balling, R .

NATURE GENETICS, 2000, 25 (04) :444-447

[4] Identification of immunological relevant phenotypes in ENU mutagenized mice [J].

Flaswinkel, H ;

Alessandrini, F ;

Rathkolb, B ;

Decker, T ;

Kremmer, E ;

Servatius, A ;

Jakob, T ;

Soewarto, D ;

Marschall, S ;

Fella, C ;

Behrendt, H ;

Ring, J ;

Wolf, E ;

Balling, R ;

de Angelis, MH ;

Pfeffer, K .

MAMMALIAN GENOME, 2000, 11 (07) :526-527

[5] CD83 expression influences CD4+ T cell development in the thymus [J].

Fujimoto, Y ;

Tu, LL ;

Miller, AS ;

Bock, C ;

Fujimoto, M ;

Doyle, C ;

Steeber, DA ;

Tedder, TF .

CELL, 2002, 108 (06) :755-767

[6] Effects of ENU dosage on mouse strains [J].

Justice, MJ ;

Carpenter, DA ;

Favor, J ;

Neuhauser-Klaus, A ;

de Angelis, MH ;

Soewarto, D ;

Moser, A ;

Cordes, S ;

Miller, D ;

Chapman, V ;

Weber, JS ;

Rinchik, EM ;

Hunsicker, PR ;

Russell, WL ;

Bode, VC .

MAMMALIAN GENOME, 2000, 11 (07) :484-488

[7] An allelic series at the PDGFαR locus indicates unequal contributions of distinct signaling pathways during development [J].

Klinghoffer, RA ;

Hamilton, TG ;

Hoch, R ;

Soriano, P .

DEVELOPMENTAL CELL, 2002, 2 (01) :103-113

[8] The structure of the protein universe and genome evolution [J].

Koonin, EV ;

Wolf, YI ;

Karev, GP .

NATURE, 2002, 420 (6912) :218-223

[9] Positive and negative regulation of T-cell activation by adaptor proteins [J].

Koretzky, GA ;

Myung, PS .

NATURE REVIEWS IMMUNOLOGY, 2001, 1 (02) :95-107

[10] Vulval development in Caenorhabditis elegans [J].

Kornfeld, K .

TRENDS IN GENETICS, 1997, 13 (02) :55-61

← 1 2 3 →