Polyaniline Langmuir-Blodgett film based cholesterol biosensor

被引：88

作者：

Matharu, Zimple ^{[1
,2
]}

Sumana, G. ^{[1
]}

Arya, Sunil K. ^{[1
]}

Singh, S. P. ^{[1
]}

Gupta, Vinay ^{[2
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Univ Delhi, Dept Phys & Astron, Delhi 110007, India

来源：

LANGMUIR | 2007年 / 23卷 / 26期

关键词：

D O I：

10.1021/la702123a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cholesterol oxidase (ChOx) has been covalently linked to Langmuir-Blodgett (LB) monolayers of polyaniline (PANI)-stearic acid (SA) prepared onto indium-tin-oxide (ITO) coated glass plates via glutaraldehyde (Glu) chemistry. These ChOx/Glu/PANI-SA LB film/ITO electrodes have been characterized by FT-IR, cyclic voltammetry, and scanning electron microscopy, respectively. The results of response measurements carried out on these bioelectrodes using linear sweep voltammetry (LSV) reveal linearity from 25 to 400 mg/dL of cholesterol concentration with sensitivity of 88.9 nA mg(-1) dL. The linear regression analysis of bioelectrode reveals standard deviation and correlation coefficient of 0.737 mu A and 0.9988, respectively. The low value of the Michaelis-Menten constant of these bioelectrodes obtained as 1.21 mM for the immobilized enzyme indicates increased interaction between ChOx and cholesterol in the PANI-SA LB film.

引用

页码：13188 / 13192

页数：5

共 33 条

[1] Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique [J].

Arya, Sunil K. ;

Solanki, Pratima R. ;

Singh, Ravindra P. ;

Pandey, Manoj K. ;

Datta, Monika ;

Malhotra, Bansi D. .

TALANTA, 2006, 69 (04) :918-926

[2] An electrochemical multienzymatic biosensor for determination of cholesterol [J].

Bongiovanni, C ;

Ferri, T ;

Poscia, A ;

Varalli, M ;

Santucci, R ;

Desideri, A .

BIOELECTROCHEMISTRY, 2001, 54 (01) :17-22

[3] Glucose oxidase electrodes of polyaniline, poly(o-toluidine) and their copolymer as a biosensor:: a comparative study [J].

Borole, DD ;

Kapadi, UR ;

Mahulikar, PP ;

Hundiwale, DG .

POLYMERS FOR ADVANCED TECHNOLOGIES, 2004, 15 (06) :306-312

[4] Co-immobilization of lactate oxidase and lactate dehydrogenase on conducting polyaniline films [J].

Chaubey, A ;

Pande, KK ;

Singh, VS ;

Malhotra, BD .

ANALYTICA CHIMICA ACTA, 2000, 407 (1-2) :97-103

[5]

Coêlho RAL, 2001, J BIOMED MATER RES, V56, P257, DOI 10.1002/1097-4636(200108)56:2<257::AID-JBM1093>3.3.CO

[6]

2-9

[7] Preparation and characterization of mixed LB films of polyaniline and cadmium arachidate [J].

Dhanabalan, A ;

Dabke, RB ;

Datta, SN ;

Kumar, NP ;

Major, SS ;

Talwar, SS ;

Contractor, AQ .

THIN SOLID FILMS, 1997, 295 (1-2) :255-259

[8] A study of Langmuir and Langmuir-Blodgett films of polyaniline [J].

Dhanabalan, A ;

Dabke, RB ;

Kumar, NP ;

Talwar, SS ;

Major, S ;

Lal, R ;

Contractor, AQ .

LANGMUIR, 1997, 13 (16) :4395-4400

[9]

DONG S, 2002, MOL BIOTECHNOL, V82, P303

[10]

FORSTER R, 2000, ELECTROANAL, V12, P716

← 1 2 3 4 →