New approaches towards fluorescence labelling of messenger RNA transcripts

被引：4

作者：

Garnier, A ^{[1
]}

Hüsken, D ^{[1
]}

Weiler, J ^{[1
]}

机构：

[1] Novartis Pharma AG, CH-4002 Basel, Switzerland

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002515

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence labelling of nucleic acids is being used for a wide range of biological applications. The performance of these techniques is dependent on fluorochrome labels with a high sensitivity and high resistance to photobleaching. Indo-cyanine dyes such as Cy3, Cy5 or Cy7 have been found to fulfil these requirements. This study describes several different RNA labelling techniques allowing for a Cy5 based detection of mRNA transcripts.

引用

页码：1181 / 1185

页数：5

共 4 条

[1] Sequence analysis by hybridization with oligonucleotide microchip: Identification of beta-thalassemia mutations [J].