Gurmarin sensitivity of sweet taste responses is associated with co-expression patterns of T1r2, T1r3, and gustducin

被引:28
作者
Shigemura, Noriatsu [1 ]
Nakao, Kazuko [1 ,2 ]
Yasuo, Toshiaki [1 ]
Murata, Yoshihiro [1 ]
Yasumatsu, Keiko [1 ]
Nakashima, Akihiko [2 ]
Katsukawa, Hideo [3 ]
Sako, Noritaka [3 ]
Ninomiya, Yuzo [1 ]
机构
[1] Kyushu Univ, Grad Sch Dent Sci, Sect Oral Neurosci, Higashi Ku, Fukuoka 8128582, Japan
[2] Kyushu Univ, Dept Orthodont, Fukuoka 8128582, Japan
[3] Asahi Univ Sch Dent, Dept Oral Physiol, Gifu 5010296, Japan
关键词
taste; sweet-; gurmarin; T1r2; T1r3; gustducin; in situ hybridization;
D O I
10.1016/j.bbrc.2007.12.146
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gurmarin (Gur) is a peptide that selectively suppresses sweet taste responses in rodents. The inhibitory effect of Gur differs among tongue regions and mouse strains. Recent studies demonstrated that co-expression levels of genes controlling sweet receptors (T1r2/T1r3 heterodimer) versus G alpha-protein, gustducin, are much lower in Gur-insensitive posterior circumvallate papillae than in Gur-sensitive anterior fungiform papillae. Here, we investigated the potential link of Gur-sensitivity with the co-expression for T1r2/T1r3 receptors and gustducin by comparing those of taste tissues of Gur-sensitive (B6, dpa congenic strains) and Gur-weakly- sensitive (BALB) strains. The results indicated that co-expression ratios among T1r2, T1r3, and gustducin in the fungiform papillae were significantly lower in Gur-weakly-sensitive BALB mice than in Gur-sensitive B6 and dpa congenic mice. This linkage between Gur-sensitivity and co-expression for T1r2/T1r3 receptors versus gustducin suggests that gustducin may be a key molecule involved in the pathway for Gur-sensitive sweet responses. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:356 / 363
页数:8
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