Direct determination of benzamides in serum by column-switching high-performance liquid chromatography

被引：27

作者：

Chiba, R ^{[1
]}

Ogasawara, A

Kubo, T

Yamazaki, H

Umino, M

Ishizuka, Y

机构：

[1] Showa Pharmaceut Univ, Machida, Tokyo 1948543, Japan

[2] Tosoh Co, Sci Instrument Div, Ayase, Kanagawa 2521123, Japan

[3] Teikyo Univ, Sch Med, Mizonokuchi Hosp, Kawasaki, Kanagawa 2130001, Japan

来源：

ANALYTICAL SCIENCES | 2003年 / 19卷 / 05期

关键词：

D O I：

10.2116/analsci.19.785

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (SIN = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.

引用

页码：785 / 789

页数：5

共 23 条

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